Performance of reduced bit-depth acquisition for optical frequency domain imaging
Performance of reduced bit-depth acquisition for optical frequency domain imaging
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DOI：
10.1364/oe.17.016957
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发表时间：
2009-09-14
期刊：
影响因子：
3.8
通讯作者：
Tearney, Guillermo J.
中科院分区：
文献类型：
--
作者：
Goldberg, Brian D.;Vakoc, Benjamin J.;Tearney, Guillermo J.
高速光学频域成像(OFDI)使生物实验室和临床医学的广域显微成像实用化成为可能。对外直接投资的成像速度以及当前系统的视场受到数据数字化和存档速度的限制，而不是系统灵敏度或激光性能的限制。该瓶颈的一种解决方案是以减少的比特深度(例如，以8比特深度而不是传统的12-14比特深度)对OFDI信号进行本机数字化，从而减少总体带宽。然而，减少比特深度获取对图像质量的影响尚未被研究。在本文中，我们使用模拟和实证研究来评估减少深度获取对对外直接投资图像质量的影响。我们表明，8位深度的图像采集允许较高的系统灵敏度，与较高位深的系统相比，仅有最小的信噪比下降。给出了活体采集的8位深度的人体冠状动脉图像，并与高位深度采集的图像进行了比较。(C)2009年美国光学学会
High-speed optical frequency domain imaging (OFDI) has enabled practical wide-field microscopic imaging in the biological laboratory and clinical medicine. The imaging speed of OFDI, and therefore the field of view, of current systems is limited by the rate at which data can be digitized and archived rather than the system sensitivity or laser performance. One solution to this bottleneck is to natively digitize OFDI signals at reduced bit depths, e. g., at 8-bit depth rather than the conventional 12-14 bit depth, thereby reducing overall bandwidth. However, the implications of reduced bit-depth acquisition on image quality have not been studied. In this paper, we use simulations and empirical studies to evaluate the effects of reduced depth acquisition on OFDI image quality. We show that image acquisition at 8-bit depth allows high system sensitivity with only a minimal drop in the signal-to-noise ratio compared to higher bit-depth systems. Images of a human coronary artery acquired in vivo at 8-bit depth are presented and compared with images at higher bit-depth acquisition. (C) 2009 Optical Society of America