A single zinc finger motif in the silencing factor REST represses the neural-specific type II sodium channel promoter.
A single zinc finger motif in the silencing factor REST represses the neural-specific type II sodium channel promoter.
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DOI：
10.1073/pnas.94.4.1177
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发表时间：
1997-02
影响因子：
11.1
通讯作者：
José Tapia-Ramirez;Bart J. L. Eggen;Maria J. Peral-Rubio;J. Toledo-Aral;J. Toledo-Aral;Gail Mandel
中科院分区：
文献类型：
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作者：
José Tapia-Ramirez;Bart J. L. Eggen;Maria J. Peral-Rubio;J. Toledo-Aral;J. Toledo-Aral;Gail Mandel
II型电压依赖性钠通道存在于神经细胞中，在那里它介导神经冲动的传播。II型钠通道基因对神经元的表达受限至少部分是由于抑制蛋白REST(也称为NRSF或XBR)与II型钠通道基因中的RE1(也称为NRSE)序列结合。先前的研究表明，包含8个GL1-Krüppel锌指基序的REST结构域介导DNA结合。缺失和GAL4融合基因分析现在揭示了位于REST的氨基和羧基末端的DNA结合域之外的抑制域。突变分析进一步确定了羧基末端区域中的单个锌指基序对于抑制II型钠通道报告基因是必不可少的。这些研究揭示了REST中的两个区域，它们可能介导与其他蛋白质的相互作用，这些蛋白质涉及限制大量基因在脊椎动物神经系统中的表达。
The type II voltage-dependent sodium channel is present in neuronal cells, where it mediates the propagation of nerve impulses. Restricted expression of the type II sodium channel gene to neurons is due, at least in part, to binding of the repressor protein REST (also termed NRSF or XBR) to the RE1 (also called NRSE) sequence in the type II sodium channel gene. Previous studies have shown that a domain in REST containing eight GL1-Krüppel zinc finger motifs mediates DNA binding. Deletional and GAL4-fusion gene analyses now reveal repressor domains that lie outside of the DNA-binding domain in both the amino and carboxyl termini of REST. Mutational analysis further identifies a single zinc finger motif in the carboxyl-terminal domain as being essential for repressing type II sodium channel reporter genes. These studies reveal two domains in REST that may mediate interactions with other proteins involved in restricting expression of a large set of genes to the vertebrate nervous system.