Notch-mediated segmentation and growth control of the Drosophila leg
Notch-mediated segmentation and growth control of the Drosophila leg
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DOI：
10.1006/dbio.1999.9273
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发表时间：
1999-06-15
影响因子：
2.7
通讯作者：
Irvine, KD
中科院分区：
文献类型：
--
作者：
Rauskolb, C;Irvine, KD
拥有分节附肢是节肢动物的一个定义性特征。通过分析功能丧失和异位表达实验，我们表明Notch信号通路在果蝇腿的分割和生长中起着重要作用。Notch的局部激活对于促进节段之间关节的形成是必要且充分的。这种分割过程需要Notch配体、Serrate和Delta以及边缘的参与。这三种蛋白质分别在发育中的腿和触角成虫盘中以片段重复的模式表达，该模式在无翅和Decapentaplegic的作用下游受到调节。我们的研究进一步表明，Notch激活对于促进腿部生长是必要的，也是足够的。我们还确定了正常腿部发育所需的Notch信号下游正向和负向调控的靶基因。总之，这些观察结果概述了腿的分割和生长的监管层次结构。Notch通路也被用于脊椎动物体节发生期间的分割，这提高了这些不同组织的分割的共同起源的可能性。(C)北京：科学出版社.
The possession of segmented appendages is a defining characteristic of the arthropods. By analyzing both loss-of-function and ectopic expression experiments, we show that the Notch signaling pathway plays a fundamental role in the segmentation and growth of the Drosophila leg. Local activation of Notch is necessary and sufficient to promote the formation of joints between segments. This segmentation process requires the participation of the Notch ligands, Serrate and Delta, as well as fringe. These three proteins are each expressed in the developing leg and antennal imaginal discs in a segmentally repeated pattern that is regulated downstream of the action of Wingless and Decapentaplegic. Our studies further show that Notch activation is both necessary and sufficient to promote leg growth. We also identify target genes regulated both positively and negatively downstream of Notch signaling that are required for normal leg development. Together, these observations outline a regulatory hierarchy for the segmentation and growth of the leg. The Notch pathway is also deployed for segmentation during vertebrate somitogenesis, which raises the possibility of a common origin for the segmentation of these distinct tissues. (C) 1999 Academic Press.