Impaired hyperactivation of human sperm in patients with infertility.
Impaired hyperactivation of human sperm in patients with infertility.
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DOI：
10.11480/jmds.510113
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发表时间：
2004-03
影响因子：
--
通讯作者：
M. Munire;Y. Shimizu;Y. Sakata;R. Minaguchi;T. Aso
中科院分区：
文献类型：
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作者：
M. Munire;Y. Shimizu;Y. Sakata;R. Minaguchi;T. Aso
过度活化和顶体反应是精子能够与卵母细胞受精的先决条件。在哺乳动物中，过度激活被定义为精子在受精地点和时间的运动模式。本实验的目的是分析过度激活的过程，并探讨其与孕激素引起的细胞内钙浓度（[Ca2+]i）升高的关系及其与不孕症的关系。患者精子获能后，与供体精子相比，高激活精子的百分比、活力、进行性运动和曲线速度（VCL）均有所下降。另一方面，线性度（LIN）增加。不孕症患者侧头移位幅度（ALH）与[Ca2+]i升高（从基线开始的峰值和平台）具有良好的相关性。这些数据表明，受损的过度激活可能参与不孕症的病理生理。
Hyperactivation and acrosome reaction are prerequisite steps for sperm to be able to fertilize an oocyte. In mammals, hyperactivation is defined as a movement pattern seen in spermatozoa at the site and time of fertilization. The objectives of the present experiments were to analyze the process of hyperactivation and to investigate its relationship with progesterone evoked intracellular calcium concentration ([Ca2+]i) increase and their implications with infertility. After capacitation the sperm from patients, when compared with donor's sperm, showed decreased percentage of hyperactivated sperm, molitily, progressive motility, and curvilinear velocity (VCL). On the other hand, the linearity (LIN) was increased. Amplitude of lateral head displacement (ALH) and [Ca2+]i increase (peak and plateau from baseline) showed good correlation in patients with infertility. These data suggest that impaired hyperactivation might be involved in the pathophysiology of infertility.