Fabrication of AIE-active fluorescent polymeric nanoparticles with red emission through a facile catalyst-free amino-yne click polymerization
Fabrication of AIE-active fluorescent polymeric nanoparticles with red emission through a facile catalyst-free amino-yne click polymerization
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DOI：
10.1016/j.dyepig.2017.12.046
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发表时间：
2018-04
影响因子：
4.5
通讯作者：
Ruming Jiang;Meiying Liu;Hongye Huang;Liucheng Mao;Qiang Huang;Y. Wen;Qian‐Yong Cao;Jianwen Tian;Xiaoyong Zhang;Yen Wei
中科院分区：
文献类型：
--
作者：
Ruming Jiang;Meiying Liu;Hongye Huang;Liucheng Mao;Qiang Huang;Y. Wen;Qian‐Yong Cao;Jianwen Tian;Xiaoyong Zhang;Yen Wei
荧光聚合物纳米颗粒作为一种新型探针，由于其光学特性和多功能潜力，在生物医学领域的应用越来越受到人们的关注。然而，大多数基于传统有机氟化物的fpn存在严重的聚集猝灭（ACQ）效应。为了克服这些问题，在较温和的实验条件下（无催化剂和室温），通过简单的氨基-炔点击聚合制备了含有聚集诱导发射（AIE）氟原（AIEgens）的两亲性荧光共聚物[Poly(peg - phe)]。聚（peg - phe）共聚物可以自组装成fpn，并且在水溶液中分散良好。荧光光谱表明，聚（peg - phe） FPNs具有较强的红色荧光、优异的光稳定性和较低的临界胶束浓度。生物学实验证实这些具有aie活性的聚乙二醇（peg - phe） FPNs对L929 细胞具有较低的细胞毒性和细胞染色性能。更重要的是，由于其高效、温和的反应条件和简单的实验操作程序，这种氨基炔点击聚合为制备健壮的生物相容性荧光纳米颗粒提供了一种简单的策略。
Fluorescent polymeric nanoparticles (FPNs) as novel probes have attracted more and more attention for biomedical applications owing to their optical properties and multifunctional potential. However, most of FPNs based on conventional organic fluorogens encounter serious aggregation-caused quenching (ACQ) effect. In this work, to overcome these problems, amphiphilic fluorescent copolymers [Poly(PEGY-PhE)] containing aggregation-induced emission (AIE) fluorogens (AIEgens) have been prepared via a facile amino-yne click polymerization under rather mild experimental conditions (catalyst-free and room temperature). The Poly(PEGY-PhE) copolymers can self-assemble into FPNs and be well dispersed in aqueous solution. Fluorescence spectra demonstrate that Poly(PEGY-PhE) FPNs possess strong red fluorescence, excellent photostability and low critical micelle concentration. The biological assays confirm that these AIE-active Poly(PEGY-PhE) FPNs possess low cytotoxicity towards L929 cells and cell dyeing performance. More importantly, this amino-yne click polymerization provides a simple strategy to prepare robust biocompatible fluorescent nanoparticles for biological imaging because of their high efficiency, mild reaction conditions and simple experimental operation procedure.