The influence of epithelia on cartilage and loose connective tissue formation by limb mesenchyme cultures.
The influence of epithelia on cartilage and loose connective tissue formation by limb mesenchyme cultures.
复制标题
肢体间充质培养物上皮对软骨和疏松结缔组织形成的影响。
DOI：
10.1016/0012-1606(81)90205-0
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发表时间：
1981
影响因子：
2.7
通讯作者：
Reiter,RS
中科院分区：
文献类型：
--
作者：
Solursh,M;Singley,CT;Reiter,RS
为了解释非软骨性肢体间充质在培养中形成广泛软骨的观察结果，研究了肢体外胚层抑制软骨形成的可能性。将小块的鹌鹑或雏鸡外胚层移植到23-34期雏鸡胚胎的微团间质培养物上。通过透射电子显微镜观察，非脊翼或其他几种上皮的存在会导致间质胶原沉积增加，细胞分化延迟，最终形成疏松的结缔组织。这种影响可以发生在具有0.1m-μ孔径的核孔过滤器和超薄的微孔过滤器上，但不会发生在标准厚度的微孔过滤器上。这种影响不依赖于接触，因为细胞突起不会穿过这些过滤器。顶端的外胚层脊具有刺激间充质生长的额外作用。这些结果支持外胚层在发育肢体内软骨化核心的形成中起直接但负面作用的观点。
In order to explain the observation that normally nonchondrogenic limb mesenchyme forms extensive cartilage in culture, the possibility that limb ectoderm inhibits chondrogenesis is examined. Small pieces of quail or chick ectoderm are grafted onto micromass cultures of wing mesenchyme from stage 23–34 chick embryos. The presence of nonridge wing or several other epithelia results in increased collagen accumulation in the underlying mesenchyme, a delay in cell differentiation, and the eventual formation of loose connective tissue, as determined by transmission electron microscopy. The influence can occur across Nuclepore filters having 0.1-μm-diameter pores and ultrathin Millipore filters, but not across Millipore filters of standard thickness. The influence is not contact dependent since cell processes do not cross these filters. The apical ectodermal ridge has the additional effect of stimulating mesenchymal outgrowth. These results support the idea that the ectoderm plays a direct but negative role in the formation of a chondrogenic core within the developing limb.