A genome scan for serum triglyceride in obese nuclear families
A genome scan for serum triglyceride in obese nuclear families
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DOI：
10.1194/jlr.m400391-jlr200
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发表时间：
2005-03-01
影响因子：
6.5
通讯作者：
Price, RA
中科院分区：
文献类型：
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作者：
Li, WD;Dong, CH;Price, RA
极度肥胖者的血清甘油三酯（TG）水平升高，表明脂质代谢和胰岛素抵抗异常。我们对 320 个核心家庭进行了血清 TG 基因组扫描，将极度肥胖和正常体重分开。对 382 个 Marshfield 微卫星标记（筛选组 11）进行了基因分型。使用家族回归和方差分量方法进行定量连锁分析。我们发现对数转换 TG 的 7q36 区域 [D7S3058，174 厘摩 (cM)，赔率对数 (LOD) = 2.98] 存在连锁。我们还在 20 号染色体（D20S164，101 cM，LOD = 2.34）、13 号染色体（111 cM，LOD = 2.00）和 9 号染色体（104 cM，LOD = 1.90）上发现了提示性连锁，并且在 1、3、5、10、12 和 22 号染色体上发现了一些较弱的趋势。在 58 名非裔美国人中在 16 号染色体上的两个基因座上观察到 LOD 得分为 3.66 和 2.62：D16S3369 (64 cM) 和 MFD466 (100 cM)。为了验证 7q36 连锁，我们添加了 60 个核心家族，并且标记 D7S3058 上的 LOD 得分增加到 3.52（经验 P < 0.002）。
Serum triglyceride (TG) levels are increased in extremely obese individuals, indicating abnormalities in lipid metabolism and insulin resistance. We carried out a genome scan for serum TG in 320 nuclear families segregating extreme obesity and normal weight. Three hundred eighty-two Marshfield microsatellite markers ( Screening Set 11) were genotyped. Quantitative linkage analyses were performed using family regression and variance components methods. We found linkage on the 7q36 region [D7S3058, 174 centimorgan (cM), Logarithm of Odds (LOD) = 2.98] for log-transformed TG. We also found suggestive linkages on chromosomes 20 (D20S164, 101 cM, LOD = 2.34), 13 (111 cM, LOD = 2.00), and 9 (104 cM, LOD = 1.90) as well as some weaker trends for chromosomes 1, 3, 5, 10, 12, and 22. In 58 African American families, LOD scores of 3.66 and 2.62 were observed on two loci on chromosome 16: D16S3369 (64 cM) and MFD466 ( 100 cM). To verify the 7q36 linkage, we added 60 nuclear families, and the LOD score increased to 3.52 (empirical P < 0.002) on marker D7S3058.