DETECTION OF PLASMA-MEMBRANE CHOLESTEROL BY FILIPIN DURING MICROVILLOGENESIS AND CILIOGENESIS IN QUAIL OVIDUCT
DETECTION OF PLASMA-MEMBRANE CHOLESTEROL BY FILIPIN DURING MICROVILLOGENESIS AND CILIOGENESIS IN QUAIL OVIDUCT
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DOI：
10.1177/33.1.3965567
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
BOISVIEUXULRICH, E
中科院分区：
文献类型：
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作者：
CHAILLEY, B;BOISVIEUXULRICH, E
用菲律宾蛋白作为膜胆固醇的探针，研究了雌激素诱导的鹌鹑输卵管纤毛发生过程中，胆固醇在顶端质膜的分布变化，并与膜内颗粒（IMP）的分布进行了比较。纤毛生长之前是微绒毛分化的第一步。微绒毛出现在富含IMP的膜结构域中，并且缺乏菲律宾胆固醇（f-c）复合物。生长中的微绒毛膜呈现f-c复合物。在纤毛生长期间，微绒毛从0.5 μ m延长至2 μ m，表明微绒毛膜不是纤毛发生的膜储库。在纤毛生长过程中，特征性的纤毛项链IMP行逐渐出现在纤毛基部。第一IMP行被组织在一个缺乏f-c复合物的膜环中，而在环中间的新轴膜显示出许多复合物。这两个不同的纤毛结构域在纤毛生长过程中保持其特异性。只有睫状体尖端比轴膜显示较少的复合物。结果表明，纤毛细胞的顶膜由不同的结构域组成，纤毛轴充满f-c复合物，缺乏IMP，纤毛项链缺乏f-c复合物，富含IMP，微绒毛膜富含IMP和f-c复合物;睫状体间膜的f-c复合物和IMP含量均较低，未分化细胞的顶膜中f-c复合物和IMP含量较低。c复合物和IMP均匀分布。
Using filipin as a probe for the presence of membrane cholesterol, the evolution of cholesterol distribution in the apical plasma membrane was studied during estrogen-induced ciliogenesis in quail oviduct and compared with the distribution of intramembrane particles (IMP). Ciliary growth is preceded by the 1st step of microvillus differentiation. Microvilli emerged in membrane domains rich in IMP and devoid of filipin-cholesterol (f-c) complexes. Growing microvillus membrane showed f-c complexes. During ciliary growth, microvilli lengthen from 0.5 to 2 .mu.m, indicating that the microvillar membrane is not a membrane reservoir for ciliogenesis. During ciliary growth, the characteristic ciliary necklace IMP rows appear progressively at the base of cilia. The 1st IMP row was organized in a membrane circlet lacking of f-c complexes, whereas the new shaft membrane in the middle of the circlet exhibited numerous complexes. These 2 different domains of the cilia kept their specificity during ciliary growth. Only the ciliary tip showed fewer complexes than the shaft membrane. The apical membrane of differentiated ciliated cells was thus composed of various domains; the ciliary shaft was full of f-c complexes and poor in IMP, the ciliary necklace was devoid of f-c complexes and rich in IMP; the microvilli membrane was rich in both IMP and f-c complexes; and the interciliary membrane was poor in both f-c complexes and IMP. The undifferentiated cells exhibited an apical membrane in which f-c complexes and IMPs are distributed homogeneously.