Comparison of culture methods on exopolysaccharide production in the submerged culture of Cordyceps militaris and process optimization
Comparison of culture methods on exopolysaccharide production in the submerged culture of Cordyceps militaris and process optimization
复制标题
DOI：
10.1111/j.1472-765x.2010.02987.x
复制
发表时间：
2011-02-01
影响因子：
2.4
通讯作者：
Zhang, B. Z.
中科院分区：
文献类型：
--
作者：
Cui, J. D.;Zhang, B. Z.
目的：为了提高北虫夏草胞外多糖的产量，研究了不同培养方法对北虫夏草深层发酵菌丝体生物量和胞外多糖产量的影响。方法与结果：设计了北虫夏草发酵生产胞外多糖的新工艺。利用中心复合设计法对两段发酵过程进行了优化。结果表明，两段发酵法生产EPS优于常规的静态培养和摇瓶培养。结果表明，EPS产生的最适条件为摇床培养140h，然后静置培养130h。在优化的两段发酵条件下，胞外多糖产量最高可达3中心点2g L-1，与静置培养和摇床培养相比，胞外多糖产量分别提高了约2个中心点3倍和1个中心点6倍。结论：本研究获得的一种新的两段培养方法具有较高的工业化生产军曹鱼胞外多糖的潜力。本研究的意义和影响：本工作所获得的基础信息是对以往关于北冰藻深层培养生产活性代谢产物的研究的补充。
Aims:To improve exopolysaccharides (EPS) production of Cordyceps militaris (C. militaris), effects of different culture method on mycelial biomass and EPS production in the submerged culture of C. militaris were investigated.Methods and Results:A new two-stage fermentation process for EPS production of C. militaris was designed in this work. Central composite design (CCD) was utilized to optimize the two-stage fermentation process. The results showed that the two-stage fermentation process for EPS production was superior to other culture method (conventional static culture and shake culture). CCD revealed that the optimum values of the test variables for EPS production were shaken for 140 h followed by 130-h static culture. The maximum EPS production reached 3 center dot 2 g l-1 under optimized two-stage culture and was about 2 center dot 3-fold and 1 center dot 6-fold in comparison with those of original static culture and shake culture.Conclusions:It was indicated that a new two-stage culture method obtained in this work possessed a high potential for the industrial production for EPS of C. militaris.Significance and Impact of the Study:The fundamental information obtained in this work is complementary to those of previous investigations on the submerged culture of C. militaris for the production of bioactive metabolites.