Activation of Ras cascade increases the mitochondrial enzyme content of respiratory competent yeast
Activation of Ras cascade increases the mitochondrial enzyme content of respiratory competent yeast
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DOI：
10.1016/s0006-291x(02)00391-1
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发表时间：
2002-05-24
影响因子：
3.1
通讯作者：
Rigoulet, M
中科院分区：
文献类型：
--
作者：
Dejean, L;Beauvoit, B;Rigoulet, M
我们研究了cAMP-蛋白激酶A途径的遗传和生理调节对乳酸盐酵母细胞线粒体生物合成的影响。具有过度激活的Ras/腺苷酸环化酶途径的酵母突变体（即，Ras 2（val 19），ira 1 Δ 2Delta）或具有蛋白激酶A的组成性下游活化（即，bcyDelta）显示线粒体酶含量增加。相反，Ras活性的丧失（即，Ras 2突变体）导致轻微下降。cAMP处理的响应突变体增加了细胞的氧化磷酸化能力，并增加了编码线粒体蛋白的核基因的转录水平。相反，线粒体DNA基因的转录水平没有变化。它的结论是Ras/cAMP/蛋白激酶A途径是控制酵母细胞中的氧化磷酸化复合物的生物合成的调节回路的一部分。(C)2002 Elsevier Science（美国）。All rights reserved.
We investigated the effects of genetic and physiological modulations of the cAMP-protein kinase A pathway on mitochondrial biogenesis of yeast cells grown on lactate. Yeast mutants with over-activated Ras/adenylate cyclase pathway (i.e., Ras2(val19), ira1Deltaira2Delta) or with a constitutive downstream activation of protein kinases A (i.e., bcyDelta) showed an increase in the mitochondrial enzyme content. In contrast, loss of Ras activity (i.e., Ras2 mutant) resulted in a slight decrease. The treatment by cAMP of a responsive mutant increased the oxidative phosphorylation capacity of cells and increased the transcript level of nuclear genes encoding for mitochondrial proteins. In contrast, the transcript level of mitochondrial DNA genes was unchanged. It is concluded that the Ras/cAMP/protein kinase A pathway is part of the regulatory circuit controlling biogenesis of the oxidative phosphorylation complexes in yeast cells. (C) 2002 Elsevier Science (USA). All rights reserved.