Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1
Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1
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DOI：
10.1104/pp.15.01483
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发表时间：
2016-02-01
期刊：
影响因子：
7.4
通讯作者：
Swiezewski, Szymon
中科院分区：
文献类型：
--
作者：
Cyrek, Malgorzata;Fedak, Halina;Swiezewski, Szymon
DOG1 （Delay of萌发1）是拟南芥（Arabidopsis thaliana）等植物种子休眠的关键调控因子。有趣的是，在许多植物物种中，DOG1的C端要么缺失，要么不保守。在这里，我们发现在拟南芥中，DOG1转录物受选择性聚腺苷酸化的影响。与此相一致的是，RNA 39加工复合体的突变体在DOG1近端聚腺苷化位点选择缺陷的同时显示出种子休眠减弱，这表明短的DOG1转录物是有功能的。近端聚腺苷化的短DOG1 mRNA在体内被翻译并与DOG1突变体互补，这一发现证实了这一点。总之，我们的研究结果表明，近端聚腺苷化DOG1 mRNA产生的短DOG1蛋白异构体在拟南芥种子休眠的建立中起着关键作用，并且在产生近端聚腺苷化功能性DOG1转录物所需的RNA 39加工复合体中具有一系列突变特征。
DOG1 (Delay of Germination 1) is a key regulator of seed dormancy in Arabidopsis (Arabidopsis thaliana) and other plants. Interestingly, the C terminus of DOG1 is either absent or not conserved in many plant species. Here, we show that in Arabidopsis, DOG1 transcript is subject to alternative polyadenylation. In line with this, mutants in RNA 39 processing complex display weakened seed dormancy in parallel with defects in DOG1 proximal polyadenylation site selection, suggesting that the short DOG1 transcript is functional. This is corroborated by the finding that the proximally polyadenylated short DOG1 mRNA is translated in vivo and complements the dog1 mutant. In summary, our findings indicate that the short DOG1 protein isoform produced from the proximally polyadenylated DOG1 mRNA is a key player in the establishment of seed dormancy in Arabidopsis and characterizes a set of mutants in RNA 39 processing complex required for production of proximally polyadenylated functional DOG1 transcript.