Insulin enhances transcription of the tyrosine aminotransferase gene in rat liver.
Insulin enhances transcription of the tyrosine aminotransferase gene in rat liver.
复制标题
胰岛素增强大鼠肝脏中酪氨酸转氨酶基因的转录。
DOI：
10.1016/0003-9861(86)90513-8
复制
发表时间：
1986
影响因子：
3.9
通讯作者：
Kenney,FT
中科院分区：
文献类型：
--
作者：
Lee,KL;Isham,KR;Johnson,A;Kenney,FT
用克隆的cDNA探针研究了胰岛素诱导大鼠肝脏酪氨酸转氨酶的机制。氨基转移酶mRNA的水平增加约四倍后，管理的激素。这种诱导的mRNA积累不需要novoprotein合成。在离体肝细胞核中的核径流转录测定表明，胰岛素对氨基转移酶基因转录具有快速和时间依赖性的刺激作用。转录增强的幅度可以完全解释mRNA的增加。我们的结论是，诱导酪氨酸转氨酶在大鼠肝脏中的胰岛素主要是一个选择性增加的转氨酶基因的转录速率的结果。
The mechanism of insulin-mediated induction of tyrosine aminotransferase in rat liver was investigated using a cloned cDNA probe. The level of aminotransferase mRNA increases about fourfold following administration of the hormone. This induced mRNA accumulation does not requirede novoprotein synthesis. Nuclear runoff transcription assays in isolated liver nuclei demonstrate that insulin has a rapid and time-dependent stimulatory effect on aminotransferase gene transcription. The magnitude of enhanced transcription can fully account for the increase in the mRNA. We conclude that the induction of tyrosine aminotransferase in rat liver by insulin is primarily a consequence of a selective increase in the rate of transcription of the aminotransferase gene.