Simultaneous Detection of α-Fetoprotein and Carcinoembryonic Antigen Based on Si Nanowire Field-Effect Transistors.
Simultaneous Detection of α-Fetoprotein and Carcinoembryonic Antigen Based on Si Nanowire Field-Effect Transistors.
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基于硅纳米线场效应晶体管的甲胎蛋白和癌胚抗原的同时检测。
DOI：
10.3390/s150819225
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发表时间：
2015-08-05
期刊：
影响因子：
--
通讯作者：
Wang T
中科院分区：
文献类型：
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作者：
Zhu K;Zhang Y;Li Z;Zhou F;Feng K;Dou H;Wang T
原发性肝癌（primary hepatic carcinoma，PHC）是世界范围内最常见的恶性肿瘤之一，可导致患者在6 ~ 20个月内死亡。在早期诊断时，通过有效的治疗可以提高生存率。甲胎蛋白（AFP）和癌胚抗原（CEA）已被确定为在PHC患者中以较高水平表达的标志物。在这项研究中，我们采用硅纳米线场效应晶体管（SiNW-FET）与聚二甲基硅氧烷（PDMS）的微流控通道，同时检测AFP和CEA在脱盐的人血清。双通道PDMS首先用于SiNW上AFP和CEA抗体的选择性修饰，而单通道PDMS提供血清中AFP和CEA的更快和更灵敏的检测。在SiNW改性过程中，使用0.1% BSA以最小化与血清的非特异性蛋白结合。AFP和CEA检测的线性动态范围分别为500 fg/mL至50 ng/mL和50 fg/mL至10 ng/mL。我们的工作证明了制造的SiNW-FET作为血清中多种肿瘤标志物的直接检测试剂盒的潜力;因此，它为PHC患者的早期诊断提供了机会，从而为PHC患者提供了更有效的治疗。
Primary hepatic carcinoma (PHC) is one of the most common malignancies worldwide, resulting in death within six to 20 months. The survival rate can be improved by effective treatments when diagnosed at an early stage. The α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) have been identified as markers that are expressed at higher levels in PHC patients. In this study, we employed silicon nanowire field-effect transistors (SiNW-FETs) with polydimethylsiloxane (PDMS) microfluidic channels to simultaneously detect AFP and CEA in desalted human serum. Dual-channel PDMS was first utilized for the selective modification of AFP and CEA antibodies on SiNWs, while single-channel PDMS offers faster and more sensitive detection of AFP and CEA in serum. During the SiNW modification process, 0.1% BSA was utilized to minimize nonspecific protein binding from serum. The linear dynamic ranges for the AFP and CEA detection were measured to be 500 fg/mL to 50 ng/mL and 50 fg/mL to 10 ng/mL, respectively. Our work demonstrates the promising potential of fabricated SiNW-FETs as a direct detection kit for multiple tumor markers in serum; therefore, it provides a chance for early stage diagnose and, hence, more effective treatments for PHC patients.