Modeling of Protrusion Phenotypes Driven by the Actin-Membrane Interaction
Modeling of Protrusion Phenotypes Driven by the Actin-Membrane Interaction
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DOI：
10.1016/j.bpj.2009.12.4311
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发表时间：
2010-04-21
影响因子：
3.4
通讯作者：
Falcke, Martin
中科院分区：
文献类型：
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作者：
Enculescu, Mihaela;Sabouri-Ghomi, Mohsen;Falcke, Martin
我们提出了一个数学模型，用于模拟的前沿动态迁移细胞之间的相互作用的弹性性能，肌动蛋白细胞骨架的架构，和膜的力学。我们的方法是基于描述的长度和附着动力学的肌动蛋白丝的板状伪足网络。它用于确定在沿着前缘的每个位置和每个时间步长处施加在膜上的总力。该模型再现了显着的状态开关突起形态动力学实验发现上皮细胞在控制条件下和细胞表达组成型活性的Rac，参与调节的信号分子板状伪足网络组装之间。该模型还提出了一个机械解释的实验失真引起的Arp 2/3和cofilin活性失调的突起形态动力学。
We propose a mathematical model for simulating the leading-edge dynamics of a migrating cell from the interplay among elastic properties, architecture of the actin cytoskeleton, and the mechanics of the membrane. Our approach is based on the description of the length and attachment dynamics of actin filaments in the lamellipodium network. It is used to determine the total force exerted on the membrane at each position along the leading edge and at each time step. The model reproduces the marked state switches in protrusion morphodynamics found experimentally between epithelial cells in control conditions and cells expressing constitutively active Rac, a signaling molecule involved in the regulation of lamellipodium network assembly. The model also suggests a mechanistic explanation of experimental distortions in protrusion morphodynamics induced by deregulation of Arp2/3 and cofilin activity.