A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L.)
A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L.)
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DOI：
10.1007/s10681-007-9431-5
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发表时间：
2007-11-01
期刊：
影响因子：
1.9
通讯作者：
Luo, Lijun
中科院分区：
文献类型：
--
作者：
Lou, Qiaojun;Chen, Liang;Luo, Lijun
冷害是影响温带高海拔地区水稻生产的重要限制因素之一。本研究利用146个微卫星标记，鉴定了水稻苗期耐寒数量性状位点。定位群体由193个双单倍体（DH）系组成，由耐寒粳稻品种（AAV002863）与冷敏籼稻品种（珍汕97b）杂交而成。通过低温处理后幼苗的成活率来评价其耐寒性。在气候室中，在6℃/10℃条件下处理7 d后，于室温下恢复阶段的第6天进行测量。DH群体的表型分布近似正态拟合，偏度和峰度均小于0.3,3个重复间差异不显著。利用复合区间作图方法，在1、2、8号染色体上鉴定出5个LOD > 4.0的主要效应qtl。5个QTL的累计贡献率为62.28%，其中1个主要QTL （LOD = 15.09）位于2号染色体RM561和RM341的两侧，解释了总表型变异的27.42%。还检测到4个显著的上位相互作用，总贡献率为20.14%。
Chilling injury is one of the most important limiting factors affecting rice production in temperate and high-elevation areas. In this study, 146 microsatellite markers were employed to identify quantitative trait loci (QTL) conferring cold tolerance at seedling stage (CTS) .The mapping population consisted of 193 doubled haploid (DH) lines, which derived from a cross between a cold-tolerant japonica variety (AAV002863) and a cold-sensitive indica cultivar (Zhenshan97B). Tolerance to cold was assessed by the survival percentage of seedlings after cold treatment. In a climate chamber, after treatment at 6 degrees C/10 degrees C for 7 d, the measurement was taken on the sixth day of the recovery stage at room temperature. The phenotypic distribution of the DH population approximately fitted normality with skewness and kurtosis less than 0.3, and the difference among the three repetitions was not significant. Five main effect QTLs were identified with LOD > 4.0 on chromosomes 1, 2, 8 using a composite interval mapping approach. The accumulated contribution of the five QTLs was 62.28%, and a major QTL (LOD = 15.09) was identified on chromosome 2 flanked by RM561 and RM341, which explained 27.42% of the total phenotypic variation. Four significant epistatic interactions were also detected with a total contribution of 20.14%.