Magnetic resonance imaging of coronary arteries and heart valves in a living mouse: Techniques and preliminary results
Magnetic resonance imaging of coronary arteries and heart valves in a living mouse: Techniques and preliminary results
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DOI：
10.1006/jmre.2000.2147
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发表时间：
2000-10-01
影响因子：
2.2
通讯作者：
Haase, A
中科院分区：
文献类型：
--
作者：
Ruff, J;Wiesmann, F;Haase, A
小鼠心脏MRI的新研究显示了高对比度的心脏图像，从而有可能在体内对小鼠进行功能性心脏研究。但是，核磁共振成像是否还能显示活体老鼠的冠状动脉和心脏瓣膜等微观结构呢？为了回答这个问题，使用有和没有流量补偿的二维和三维梯度回波序列对冠状动脉进行成像。为了提高小鼠心脏成像的信噪比，对鸟笼式谐振器进行了优化。通过流入效应实现血液与心肌的对比。用多重重叠薄板技术获得的分段三维FLASH序列效果最好。该技术实现了100厘米的各向同性分辨率。可以看到左冠状动脉直至心尖。这是通过小鼠心脏的短轴视图和3D表面重建来证明的。用三维方法也可以看到四个心脏瓣膜。(C) 2000年学术出版社。
New investigations in MRI of a mouse heart showed high-contrast cardiac images and thereby the possibility of doing functional cardiac studies of in vivo mice. But is MRI, in addition, capable of visualizing microstructures such as the coronary arteries and the heart valves of a living mouse? To answer this question, 2D and 3D gradient echo sequences with and without flow compensation were used to image the coronary arteries. To increase signal-to-noise ratio, a birdcage resonator was optimized for mouse heart imaging. Contrast between blood and myocardium was achieved through the inflow effect. A segmented three-dimensional FLASH sequence acquired with a multiple overlap thin slab technique showed the best results. With this technique an isotropic resolution of 100 Ccm was achieved. The left coronary artery could be visualized up to the apex of the heart. This is demonstrated with short axis views and 3D surface reconstructions of the mouse heart. The four cardiac valves were also visible with the 3D method. (C) 2000 Academic Press.