Culture environment-induced pluripotency of SACK-expanded tissue stem cells.
Culture environment-induced pluripotency of SACK-expanded tissue stem cells.
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培养环境诱导 SACK 扩增的组织干细胞的多能性。
DOI：
10.1155/2011/312457
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发表时间：
2011
影响因子：
--
通讯作者：
Sherley,JamesL
中科院分区：
文献类型：
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作者：
Paré,Jean-François;Sherley,JamesL
以前提高细胞重编程以产生诱导多能干细胞(IPSCs)的效率的努力主要集中在转录因子和小分子组合上。在这里，我们报告了我们关注的结果，而不是针对重新编程的细胞的表型。我们发现，通过抑制不对称细胞动力学(SACK)方法获得的成年小鼠胰腺组织干细胞只需在生产和维持多潜能干细胞的条件下进行培养就可以获得更高的潜能。此外，补充促进对称性自我更新的麻袋制剂黄嘌呤，显著提高了获得多能性特性的效率和程度。在移植分析中，克隆重编程的胰腺干细胞产生生长缓慢的肿瘤，其组织衍生自所有三个胚胎生殖层。在没有外源转录因子转导的情况下，这种多能性的获得支持了组织干细胞易于进行细胞重新编程的概念，特别是当对称性自我更新时。
Previous efforts to improve the efficiency of cellular reprogramming for the generation of induced pluripotent stem cells (iPSCs) have focused mainly on transcription factors and small molecule combinations. Here, we report the results of our focus instead on the phenotype of the cells targeted for reprogramming. We find that adult mouse pancreatic tissue stem cells derived by the method of suppression of asymmetric cell kinetics (SACK) acquire increased potency simply by culture under conditions for the production and maintenance of pluripotent stem cells. Moreover, supplementation with the SACK agent xanthine, which promotes symmetric self‐renewal, significantly increases the efficiency and degree of acquisition of pluripotency properties. In transplantation analyses, clonal reprogrammed pancreatic stem cells produce slow‐growing tumors with tissue derivative of all three embryonic germ layers. This acquisition of pluripotency, without transduction with exogenous transcription factors, supports the concept that tissue stem cells are predisposed to cellular reprogramming, particularly when symmetrically self‐renewing.