Analysis of the codon bias in E. coli sequences.
Analysis of the codon bias in E. coli sequences.
复制标题
DOI：
10.1080/07391102.1984.10507593
复制
发表时间：
1984-12
影响因子：
4.4
通讯作者：
R. Blake;P. Hinds
中科院分区：
文献类型：
--
作者：
R. Blake;P. Hinds
根据平均密码子偏好的性质和水平，对来自大肠杆菌的 53 个基因序列（包含 18,288 个阅读框三联体）进行了表征。平均偏好的分布是双峰的，大约一半的基因平均仅使用 36 个密码子，其余的仅使用 42 个密码子。密码子偏倚水平、tRNA 群体和蛋白质产物丰度之间存在高度相关性，表明细胞利用偏倚模式来生产水平差异很大的基因产物。这种关系在参与转录和翻译成分生产的基因中尤其引人注目。总体而言，这些过程的基因产生的蛋白质比基因组中平均水平多五倍，并且使用的密码子少了大约五个。因此，RNA 聚合酶基因中发现的非常高的密码子偏倚为这些成分和 RNA 聚合酶的协调合成提供了一种简单的自源机制。在编码序列互补体的三联体中也发现了令人惊讶的高水平密码子概率。这显然是由于编码序列的进化分散和/或信使RNA二级结构水平增加的需要。
Fifty-three gene sequences from E. coli containing 18,288 reading frame triplets have been characterized according to the nature and level of average codon preference. The distribution of average preferences is bimodal, with approximately half the genes using an average of only 36 codons, and the remainder just 42 codons. There is a high correlation between the level of codon bias, the tRNA population and the abundance of protein product, indicating biased patterns are exploited by the cell for the production of widely different levels of gene product. This relationship is especially striking in genes involved in the production of components for transcription and translation. Overall, the genes for these processes generate some five-fold more protein than the average in the genome, and use about five fewer codons. The very high codon bias found in the RNA polymerase gene thus provides a simple, autogenous mechanism for the coordinate synthesis of these components and RNA polymerase. A surprisingly high level of codon probability is also found in triplets of the complement of coding sequences. This is apparently due to the evolutionary dispersion of coding sequences and/or the requirement for increased levels of secondary structure in messenger RNAs.