High-performance liquid chromatography using spherical aggregates of hydroxyapatite micro-crystals as adsorbent.
High-performance liquid chromatography using spherical aggregates of hydroxyapatite micro-crystals as adsorbent.
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使用羟基磷灰石微晶球形聚集体作为吸附剂的高效液相色谱法。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
H. Monma
中科院分区：
文献类型：
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作者：
T. Kawasaki;W. Kobayashi;K. Ikeda;S. Takahashi;H. Monma
建立了以羟基磷灰石（HA）微晶球形聚集体为吸附剂的高效液相色谱法（HPLC）；通过使用几种类型的蛋白质进行了初步性能测试。与先前开发的HPLC板状HA填充柱相比，球形HA填充柱显示出相当高的色谱分辨率，尽管柱长大大缩短了0.5-3 cm。但是，后一种柱所产生的压力要远远高于前一种柱所产生的压力。
High-performance liquid chromatography (HPLC) using spherical aggregates of hydroxyapatite (HA) microcrystals as adsorbent has been developed; preliminary performance tests were carried out by using several types of protein. In comparison with previously developed plate-like HA packed columns for HPLC, spherical HA packed columns show considerably high chromatographic resolutions in spite of extremely reduced column lengths of 0.5-3 cm. The pressure generated by the latter columns is much higher than that generated by the former, however.