A repressible female-specific lethal genetic system for making transgenic insect strains suitable for a sterile-release program
A repressible female-specific lethal genetic system for making transgenic insect strains suitable for a sterile-release program
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DOI：
10.1073/pnas.140142697
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发表时间：
2000-07-18
影响因子：
11.1
通讯作者：
Scott, MJ
中科院分区：
文献类型：
--
作者：
Heinrich, JC;Scott, MJ
我们在黑腹果蝇中建立了一个四环素抑制雌性特异性致死遗传系统，该系统的一个组成部分是由脂肪体控制的四环素控制的反激活子基因和来自卵黄蛋白1基因的雌性特异性转录增强子。另一个组成部分是隐藏在四环素反应元件控制下的促凋亡基因。携带这两种成分的菌株的雄性和雌性在添加四环素的培养基上都能存活，但在正常培养基上只有雄性存活。具有这种特性的菌株将是昆虫绝育释放计划的理想选择，这种计划在只释放雄性昆虫的情况下最有效。
We have developed a tetracycline-repressible female-specific lethal genetic system in the vinegar fly Drosophila melanogaster, One component of the system is the tetracycline-controlled transactivator gene under the control of the fat body and female-specific transcription enhancer from the yolk protein 1 gene. The other component consists of the proapoptotic gene hid under the control of a tetracycline-responsive element. Males and females of a strain carrying both components are viable on medium supplemented with tetracycline, but only males survive on normal medium. A strain with such properties would be ideal for a sterile-insect release program, which is most effective when only males are released in the field.