Self-Assembly of DNA Nanostructures in Different Cations.
Self-Assembly of DNA Nanostructures in Different Cations.
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DOI：
10.1002/smll.202300040
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发表时间：
2023-06
期刊：
影响因子：
13.3
通讯作者：
Arlin Rodriguez;Dhanush Gandavadi;Johnsi Mathivanan;Tingjie Song;B. Madhanagopal;Hannah Talbot;Jia Sheng;Xing Wang;A. Chandrasekaran
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文献类型：
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作者：
Arlin Rodriguez;Dhanush Gandavadi;Johnsi Mathivanan;Tingjie Song;B. Madhanagopal;Hannah Talbot;Jia Sheng;Xing Wang;A. Chandrasekaran
DNA的可编程特性允许构建定制设计的静态和动态纳米结构，而组装条件通常需要高浓度的镁离子，这限制了它们的应用。在DNA纳米结构组装测试的其他溶液条件下，迄今为止只使用了一组有限的二价和单价离子（通常是Mg2+和Na+）。在这里，我们使用不同尺寸的纳米结构研究了DNA纳米结构在各种离子中的组装：双交叉基序（76 bp），三点星基序（~134 bp）， DNA四面体（534 bp）和DNA折纸三角形（7221 bp）。我们展示了这些结构在Ca2+， Ba2+, Na+， K+和Li+中的成功组装，并使用凝胶电泳和原子力显微镜对DNA折纸三角形的视觉确认提供了定量的组装产量。我们进一步表明，与在二价离子（Mg2+， Ca2+和Ba2+）中组装的结构相比，在一价离子（Na+， K+和Li+）中组装的结构表现出高达10倍的核酸酶抗性。我们的工作为广泛的具有增强生物稳定性的DNA纳米结构提供了新的组装条件。
The programmable nature of DNA allows the construction of custom-designed static and dynamic nanostructures, and assembly conditions typically require high concentrations of magnesium ions that restricts their applications. In other solution conditions tested for DNA nanostructure assembly, only a limited set of divalent and monovalent ions are used so far (typically Mg2+ and Na+ ). Here, we investigate the assembly of DNA nanostructures in a wide variety of ions using nanostructures of different sizes: a double-crossover motif (76 bp), a three-point-star motif (~134 bp), a DNA tetrahedron (534 bp) and a DNA origami triangle (7221 bp). We show successful assembly of a majority of these structures in Ca2+ , Ba2+ , Na+ , K+ and Li+ and provide quantified assembly yields using gel electrophoresis and visual confirmation of a DNA origami triangle using atomic force microscopy. We further show that structures assembled in monovalent ions (Na+ , K+ and Li+ ) exhibit up to a 10-fold higher nuclease resistance compared to those assembled in divalent ions (Mg2+ , Ca2+ and Ba2+ ). Our work presents new assembly conditions for a wide range of DNA nanostructures with enhanced biostability.