Predictors of phthalate metabolites in urine and follicular fluid and correlations between urine and follicular fluid phthalate metabolite concentrations among women undergoing in vitro fertilization.
Predictors of phthalate metabolites in urine and follicular fluid and correlations between urine and follicular fluid phthalate metabolite concentrations among women undergoing in vitro fertilization.
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接受体外受精的女性尿液和卵泡液中邻苯二甲酸酯代谢物的预测因素以及尿液和卵泡液邻苯二甲酸酯代谢物浓度之间的相关性
DOI：
10.1016/j.envres.2020.109295
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发表时间：
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影响因子：
8.3
通讯作者：
Yu-Feng LI
中科院分区：
文献类型：
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作者：
Yang-Cheng Yao;Yao-Yao Du;Yi-Xing Wang;Tao-Ran Deng;Chong Liu;Xue-Mei Teng;Xiang Hua;Xiao-Qiong Yuan;Na Guo;Li Yin;Qiang Zeng;Yu-Feng LI
背景卵泡液中邻苯二甲酸酯类代谢物可能对正常卵泡发生有负面影响，但对体外受精(IVF)患者尿液和卵泡液中邻苯二甲酸酯类代谢物浓度的预测指标及其与邻苯二甲酸酯类代谢物浓度之间的关系了解较少。通过直接访谈获得有关人口统计特征、个人护理产品使用和塑料材料接触的信息。采用高效液相色谱-串联质谱法测定尿液和水样中8种邻苯二甲酸酯类代谢物的浓度。通过Spearman相关和线性回归分析尿液和FF样本中代谢物浓度的相关性。采用广义线性回归分析尿液和甲醛中邻苯二甲酸乙酯代谢物浓度的预测因素。结果尿液和尿液中邻苯二甲酸单乙酯和邻苯二甲酸单(2-乙基-5-氧基己基)酯之间存在弱到中度的相关性(相关系数：邻苯二甲酸单乙酯，0.350；邻苯二甲酸单(2-乙基-5-氧基己基)酯，0.377)；其他代谢物与尿液中的邻苯二甲酸酯代谢物浓度无相关性。尿代谢物浓度在确定FF代谢物浓度方面的预测能力一致较低，R2≤为0.113。体重指数(BMI)和教育程度与某些代谢物的尿液浓度呈负相关。较高的家庭收入、48小时内饮用瓶装饮料以及使用沐浴露和肥皂经常与较高的尿代谢物浓度相关。体重指数、家庭收入高、48小时内使用一次性塑料杯与尿液和尿液中邻苯二甲酸酯代谢物浓度相关。结论尿液和尿液中邻苯二甲酸酯代谢物浓度因社会人口学特征和生活方式因素而异。尿中邻苯二甲酸盐代谢产物浓度可能不适合用于评估卵巢邻苯二甲酸盐暴露。
BackgroundPhthalate metabolites in follicular fluid (FF) may negatively affect normal folliculogenesis; however, the predictors of phthalate metabolite concentrations in urine and FF and relationships between urine and FF phthalate metabolite concentrations among women undergoing in vitro fertilization (IVF) are poorly understood.ObjectiveTo investigate predictors of phthalate metabolites in urine and FF and correlations between urine and FF phthalate metabolite concentrations among women undergoing IVF.MethodWe recruited 305 women seeking infertility treatment at a reproductive center in Wuhan, China, from October to November 2016. Information regarding demographic characteristics, personal care product use and plastic material contact was obtained through direct interviews. Concentrations of 8 phthalate metabolites in urine and FF samples were measured using high-performance liquid chromatography and tandem mass spectrometry. Associations regarding metabolite concentrations in urine and FF samples were analysed by Spearman's correlation and linear regression. Generalized linear regression was used to examine potential predictors of phthalate metabolite concentrations in urine and FF.ResultsWeak to moderate associations between urine and FF samples were found for monoethyl phthalate (MEP) and mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) (correlation coefficient: MEP, 0.350; MEOHP, 0.377); no associations were observed for other metabolites. The predictive powers of urinary metabolite concentrations in determining FF metabolite concentrations were uniformly low, with R2≤ 0.113. Body mass index (BMI) and educational level were inversely associated with the urinary concentrations of certain metabolites. Higher household income, intake of bottled drinks within 48 h, and use of shower gel and soap were frequently associated with higher urinary metabolite concentrations. BMI, higher household income and use of disposable plastic cups within 48 h were associated with higher metabolite concentrations in FF.ConclusionPhthalate metabolite concentrations in urine and FF vary according to sociodemographic characteristics and lifestyle factors. Phthalate metabolite concentrations in urine may not be appropriate for estimating ovary phthalate exposure.