Autonomic cardiovascular control in methyl-CpG-binding protein 2 (Mecp2) deficient mice
Autonomic cardiovascular control in methyl-CpG-binding protein 2 (Mecp2) deficient mice
复制标题
DOI：
10.1016/j.autneu.2007.04.007
复制
发表时间：
2007-10-30
影响因子：
2.7
通讯作者：
Thong, Tran
中科院分区：
文献类型：
--
作者：
Bissonnette, John M.;Knopp, Sharon J.;Thong, Tran
甲基化CpG结合蛋白2是一种参与基因沉默的转录因子。它在大多数Rett综合征病例中发生突变。据报道，这种X连锁神经发育障碍涉及自主心血管调节异常。作为理解这些异常的基础的第一步，我们已经表征了Mecp 2缺陷小鼠的自主心血管功能。使用遥测技术在自由活动的动物中记录动脉压力波。Mecp 2（+/+）和Mecp 2（+/-）动物的基线血压和脉搏间期（PI）以及心率变异性（HRV）指数：PI的标准差（SDNN）、涵盖90% PI的范围（PI 90）和相邻PI的标准差（SDSD）相似。平均动脉压（MAP）和PI在频域的频谱分析显示，在低频I（LF 1，08-0.4 Hz）、低频2（LF 2，0.4-1.0 Hz）、中频（ME，1-3 Hz）和高频（HF，3.0-10.0 Hz）波段的相对功率相似。用阿托品或心得安阻断自主神经以及将环境温度升高至32摄氏度导致血压、PI和HRV的变化，这些变化在菌株之间没有差异。阿托品、普萘洛尔和升高的温度导致MAP和PI光谱功率的类似变化。通过静脉注射硝普钠，然后注射苯异丙胺，测试压力感受器功能。最大增益没有不同。这些结果确实揭示了Mecp 2缺陷型小鼠基因型中自主心血管调节的任何紊乱。(c)2007 Elsevier B. V.保留所有权利。
Methyl-CpG-binding protein 2 is a transcription factor that is involved in gene silencing. It is mutated in the majority of cases of Rett syndrome. This X-linked neurodevelopmental disorder is reported to involve abnormalities in autonomic cardiovascular regulation. As an initial step in understanding the basis for these abnormalities we have characterized autonomic cardiovascular function in Mecp2 deficient mice. Arterial pressure waves were recorded in freely moving animals using telemetry. Baseline blood pressure and pulse interval (PI) as well as indices of heart rate variability (HRV): standard deviation of PI (SDNN), range encompassing 90% of PIs (PI90) and standard deviation of adjacent PIs (SDSD) were similar in Mecp2(+/+) and Mecp2(+/-) animals. Spectral analysis of mean arterial pressure (MAP) and PI in the frequency domain showed similar relative power in low frequency I (LF1, 08-0.4 Hz), low frequency 2 (LF2, 0.4-1.0 Hz), middle frequency (ME, 1-3 Hz) and high frequency (HF, 3.0-10.0 Hz) bands. Autonomic blockade with atropine or propranolol as well as elevation in ambient temperature to 32 degrees C resulted in changes in blood pressure, PI and HRV that did not differ between the strains. Atropine, propranolol and elevated temperature resulted in similar changes in both MAP and PI spectral power. Baroreceptor function was tested using intravenous injections of nitroprusside followed by phenylephrine. Maximum gain was not different. These results do reveal any disturbance of autonomic cardiovascular regulation in the Mecp2 deficient mouse genotype. (c) 2007 Elsevier B.V. All rights reserved.