Suppression of HBXIP Reduces Cell Proliferation, Migration and Invasion In Vitro, and Tumorigenesis In Vivo in Human Urothelial Carcinoma of the Bladder
Suppression of HBXIP Reduces Cell Proliferation, Migration and Invasion In Vitro, and Tumorigenesis In Vivo in Human Urothelial Carcinoma of the Bladder
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DOI：
10.1089/cbr.2016.2038
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发表时间：
2016-11-01
影响因子：
3.4
通讯作者：
Liu, Shuangping
中科院分区：
文献类型：
--
作者：
Li, Xiaogang;Liu, Shuangping
乙肝X-相互作用蛋白(HBXIP)在多种类型的人类肿瘤中均有高表达，但HBXIP在膀胱尿路上皮癌(UCB)中的表达状况尚未见报道。在这项研究中，作者使用实时定量聚合酶链式反应和Western印迹来检测HBXIP在脐带血及邻近组织中的表达。HBXIP在脐带血组织中的表达明显增强。此外，他们还表明，抑制HBXIP诱导了T24细胞的细胞周期停滞和细胞凋亡增加。此外，抑制HBXIP也降低了T24和PC3细胞的增殖、迁移和侵袭。更重要的是，作者发现抑制HBXIP减少了体内的肿瘤形成，这表明HBXIP在脐带血进展中起着重要作用。这些数据首次表明HBXIP作为一种癌蛋白在脐带血中发挥作用，提示HBXIP可能成为治疗脐带血的潜在的新的治疗靶点。
Hepatitis B X-interacting protein (HBXIP) has been found overexpressed in several types of human cancer, however, the status of HBXIP expression in urothelial carcinoma of the bladder (UCB) has not been explored. In this study, the authors used real-time polymerase chain reaction and Western blot to test the expression of HBXIP in UCB and adjacent tissues. The expression of HBXIP was significantly increased in UCB tissues. In addition, they showed that suppression of HBXIP induced cell cycle arrest and increased cell apoptosis in T24 cells. Also, suppression of HBXIP also decreased T24 and PC3 cell proliferation, migration, and invasion. More importantly, the authors found that inhibition of HBXIP reduced the tumorigenesis in vivo, suggesting that HBXIP plays an important role in UCB progression. These data for the first time showed that HBXIP acts as an oncoprotein in UCB, suggesting that HBXIP may become a potential novel therapeutic target for the treatment of UCB.