Detection of PrPSc in rectal biopsy and necropsy samples from sheep with experimental scrapie
Detection of PrPSc in rectal biopsy and necropsy samples from sheep with experimental scrapie
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DOI：
10.1016/j.jcpa.2005.08.001
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发表时间：
2006-02-01
影响因子：
0.8
通讯作者：
Ulvund, MJ
中科院分区：
文献类型：
--
作者：
Espenes, A;Press, CM;Ulvund, MJ
痒病的诊断是基于疾病相关的朊病毒蛋白（Pi-Pc）在大脑或活体动物中易于接近的周围淋巴样组织中的表现。存在于直肠线的淋巴组织很容易从绵羊身上获得，而不需要麻醉。在羊经口感染瘙痒病感染的脑组织中，研究了PrPSc在这些组织中的存在。使用的方法包括免疫组织化学和组织印迹活检和死后材料。在感染后10个月，即大约从出现早期临床症状的时间开始，在与瘙痒病高易感性相关的PrP基因型动物中检测到PrPSc。在直肠黏膜中，PrPSc见于淋巴滤泡和分散在固有层的细胞中，常在隐窝上皮附近，有时在隐窝上皮内。采用Western blotting方法，将相当于8 mg组织的材料电泳后，在PrP基因型为VRQ/VRQ的绵羊直肠活检样品中检测到PrPSc。本研究表明，直肠活检样本应证明对绵羊痒病的诊断有用。(c) 2005 Elsevier Ltd版权所有。
Scrapie diagnosis is based on the demonstration of disease-associated prion protein (Pi-Pc) in brain or, in the live animal, in readily accessible peripheral lyniphoid tissue. Lymphatic tissues present at the rectoanal line were readily obtained from sheep without the need for anaesthesia. The presence of PrPSc in Such tissue was investigated in sheep infected orally with scrapie-infected brain material. The methods used consisted of immunohistochemistry and histoblotting on biopsy and post-mortem material. PrPSc was detected in animals with PrP genotypes associated with high susceptibility to scrapie from 10 months after infection, i.e., from about the time of appearance of early clinical signs. In the rectal mucosa, PrPSc was found in lymphoid follicles and in cells scattered in the lamina propria, often near and sometimes in the crypt epithelium. By Western blotting, PrPSc was detected in rectal biopsy samples of Sheep With the PrP genotype VRQ/VRQ after electrophoresis of material equivalent to 8 mg of tissue. This Study indicated that rectal biopsy samples should prove useful for the diagnosis of scrapie in sheep. (c) 2005 Elsevier Ltd. All rights reserved.