3D reconstruction in electron microscopy using ART with smooth spherically symmetric volume elements (blobs)
3D reconstruction in electron microscopy using ART with smooth spherically symmetric volume elements (blobs)
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DOI：
10.1016/s0304-3991(97)00127-7
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发表时间：
1998-04-01
期刊：
影响因子：
2.2
通讯作者：
Carazo, JM
中科院分区：
文献类型：
--
作者：
Marabini, R;Herman, GT;Carazo, JM
代数重建技术（ART）是求解线性方程组的迭代方法。它们已用于断层扫描中，从投影恢复物体。在这项工作中，我们应用一种ART方法，其中用于描述物体的基函数不是基于体素，而是更为平滑的名为“斑点”的函数。这项工作中所研究的数据采集遵循所谓的“锥形倾斜几何结构”，这种结构在生物大分子的三维电子显微镜的许多应用中普遍使用。我们仔细地将基于斑点的ART的性能与一种目前著名的三维（3D）重建算法（加权反投影）进行比较，使用一种方法为一种算法相对于另一种算法在特定任务上的相对优越性的论断赋予统计显著性水平。我们得出的结论是，基于斑点的ART能产生高质量的重建结果，特别是在恢复“垂直”方向的特征方面优于加权反投影。对于本文推荐的具体实现方式，ART的计算成本比加权反投影（WBP）的计算成本几乎小一个数量级。（C）1998年，爱思唯尔科学出版公司。版权所有。
Algebraic reconstruction techniques (ART) are iterative procedures for solving systems of linear equations. They have been used in tomography to recover objects from their projections. In this work we apply an ART approach in which the basis functions used to describe the objects are not based on voxels, but are much smoother functions named "blobs", The data collection studied in this work follows the so-called "conical tilt geometry" that is commonly used in many applications of three-dimensional electron microscopy of biological macromolecules. The performance of ART with blobs is carefully compared with a currently well-known three dimensional (3D) reconstruction algorithm (weighted backprojection) using a methodology which assigns a level of statistical significance to a claim of relative superiority of one algorithm over another for a particular task. The conclusion we reach is that ART with blobs produces high-quality reconstructions and is, in particular, superior to weighted backprojection in recovering features along the "vertical" direction. For the exact implementation recommended in this paper, the computational costs of ART are almost an order of magnitude smaller than those of WBP. (C) 1998 Elsevier Science B.V. All rights reserved.