The FLOWERING LOCUS T/TERMINAL FLOWER 1 family in Lombardy poplar
The FLOWERING LOCUS T/TERMINAL FLOWER 1 family in Lombardy poplar
复制标题
DOI：
10.1093/pcp/pcn010
复制
发表时间：
2008-03-01
影响因子：
4.9
通讯作者：
Kotoda, Nobuhiro
中科院分区：
文献类型：
--
作者：
Igasaki, Tomohiro;Watanabe, Yumiko;Kotoda, Nobuhiro
在一些植物物种中，FLOWERLOCUS T（FT）和MATERIAL FLOWER 1（TFL 1）家族中的基因已被证明在营养生长和生殖生长之间的转换的控制中是重要的。我们从伦巴第白杨（Populus nigra var. italica Koehne）。FT/TFL 1家族成员的序列分析显示，在家族成员之间以及拟南芥、番茄和葡萄藤中相同家族的成员的编码区内具有相当大的同源性。此外，该家族的成员在所有四个物种的研究显示一个共同的外显子内含子组织。系统发育分析显示，这些基因属于四个不同的分支：两个属于TFL 1分支;五个属于FT分支;每个属于FT和TFL 1的母亲和FT和TFL 1的兄弟分支。TFL 1进化枝中的一个基因PnTFL 1在营养分生组织中表达，异位表达PnTFL 1的转基因拟南芥具有晚开花表型。FT进化枝中两个基因PnFT 1和PnFT 2的表达模式表明它们在促进开花中发挥作用，异位表达PnFT 1或PnFT 2的转基因拟南芥具有早花表型。
Genes in the FLOWERING LOCUS T (FT) and TERMINAL FLOWER 1 (TFL1) family have been shown to be important in the control of the switch between vegetative and reproductive growth in several plant species. We isolated nine members of the FT/TFL1 family from Lombardy poplar (Populus nigra var. italica Koehne). Sequence analysis of the members of the FT/TFL1 family revealed considerable homology within their coding regions both among family members and to the members of the same family in Arabidopsis, tomato and grapevine. Moreover, members of this family in all four species examined display a common exonintron organization. Phylogenetic analysis revealed that the genes fall into four different clades: two into the TFL1 clade; five into the FT clade; and one each into the MOTHER OF FT AND TFL1 and BROTHER OF FT AND TFL1 clades. One gene in the TFL1 clade, PnTFL1, is expressed in vegetative meristems, and transgenic Arabidopsis that ectopically expressed PnTFL1 had a late-flowering phenotype. The expression patterns of two genes in the FT clade, PnFT1 and PnFT2, suggested a role for them in the promotion of flowering, and transgenic Arabidopsis that ectopically expressed either PnFT1 or PnFT2 had an early-flowering phenotype.