Solid-state Fermentation on Poplar Sawdust and Corncob Wastes for Lignocellulolytic Enzymes by Different Pleurotus ostreatus Strains
Solid-state Fermentation on Poplar Sawdust and Corncob Wastes for Lignocellulolytic Enzymes by Different Pleurotus ostreatus Strains
复制标题
不同平菇菌株固态发酵杨木锯末和玉米芯废弃物生产木质纤维素酶
DOI：
10.15376/biores.15.3.4982-4995
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发表时间：
2020-08-01
期刊：
影响因子：
1.5
通讯作者：
Bian, Lu-Sen
中科院分区：
文献类型：
--
作者：
Han, Mei-Ling;An, Qi;Bian, Lu-Sen
本研究以不同的木质纤维素酶解物为诱导物，采用固态发酵生产木质纤维素酶。通过测定漆酶、羧甲基纤维素酶和木聚糖酶活性对平菇菌株进行了鉴定。首次对野生菌株和栽培菌株的木质纤维素酶的分泌潜力进行了分析。野生菌株和栽培菌株在不同的木质纤维原料固态发酵中表现出独特的分泌木质纤维素酶的能力。野生平菇菌株对玉米芯的漆酶分泌和木聚糖酶活性有较好的选择性，而栽培菌株对杨树木屑有较好的选择性。野生菌株和栽培菌株表现出对杨树木屑分泌CMCase活性的一致偏好。野生菌株在短时间内获得了最大的水解酶活性，具有较大的优势。杨树木屑和玉米芯有利于野生菌株和栽培菌株分泌漆酶，有利于固态发酵漆酶的快速积累。此外，连续、稳定的漆酶生产是杨树木屑固态发酵的一个极其重要的优势，特别是在野生菌株中。这些发现有助于选择与合适的木质纤维材料相对应的合适的菌株。还可以实现工业化木质纤维素解酶一体化生产的优化。
Solid state fermentation with different lignocellulolytic materials as inducers was used for lignocellulolytic enzyme production in this study. Pleurotus ostreatus strains were assessed by measuring laccase, CMCase, and xylanase activities. The secretion potential of the lignocellulolytic enzymes by wild and cultivated strains was analyzed for the first time. The wild and cultivated strain showed their unique capacities for secreting lignocellulolytic enzymes on solid-state fermentation with different lignocellulosic materials. The wild P. ostreatus strain preferred corncob for the secretion of laccase and xylanase activity, but the cultivated strain preferred poplar sawdust. The wild strain and cultivated strain showed a consistent preference for poplar sawdust for the secretion of CMCase activity. The wild strain was advantageous because it achieved the maximum hydrolytic enzyme activities within a short time period. Poplar sawdust and corncob were conducive to laccase secretion by the wild or cultivated strains and the rapid accumulation of laccase on solid-state fermentation. Additionally, continuous, stable laccase production was an extremely important advantage by solid-state fermentation of poplar sawdust, particularly in the wild strain. These findings are helpful in selecting the appropriate strain that corresponds to suitable lignocellulosic materials. The optimization of integrated industrial lignocellulolytic enzyme production can also be achieved.