Large animal models for stem and progenitor cell analysis.
Large animal models for stem and progenitor cell analysis.
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DOI：
10.1002/0471142735.im22a01s69
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发表时间：
2005-11-01
影响因子：
--
通讯作者：
Dunbar, Cynthia E
中科院分区：
文献类型：
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作者：
Donahue, Robert E;Kuramoto, Ken;Dunbar, Cynthia E
已经证明，将关于造血的理解，特别是来自啮齿动物模型或体外人细胞模型的造血干细胞（HSC）外推到人中的应用是非常困难的。鉴于啮齿动物和人类造血生理学之间的差异以及缺乏真正的HSC体外测定，这并不奇怪。因此，遗传和细胞疗法的转化临床前开发依赖于实际和明确定义的大型动物模型的利用。本章将介绍最常用的模式物种，包括猕猴、狒狒、狗、猫和羊，并解释每种模式的独特优势和局限性。将包括通过消融细胞和基因治疗程序支持猕猴的具体协议，以向研究人员介绍维持专用于高强度实验的大型动物设施所需的资源和支持类型，并向研究人员介绍可能的程序类型。
Extrapolation of an understanding regarding hematopoiesis and, in particular, hematopoietic stem cells (HSCs) from rodent models or in vitro human cell models to applications in humans has proven very difficult. This is not surprising, given the differences between rodent and human hematopoietic physiology and the lack of true in vitro assays for HSCs. Therefore, translational preclinical development of genetic and cellular therapies is dependent on the utilization of practical and well-defined large animal models. This chapter will introduce the most commonly used model species, including macaques, baboons, dogs, cats, and sheep, and explain the particular advantages and limitations of each. Specific protocols for the support of macaques through ablative cell and gene therapy procedures will be included to introduce investigators to the types of resources and support required to maintain a large animal facility dedicated to high-intensity experimentation, and also to introduce investigators to the types of procedures that are possible.