Oryzalin as an Efficient Agent for Chromosome Doubling of Haploid Apple Shoots in vitro
Oryzalin as an Efficient Agent for Chromosome Doubling of Haploid Apple Shoots in vitro
复制标题
安磺灵作为体外单倍体苹果芽染色体加倍的有效剂
DOI：
10.1111/j.1439-0523.1994.tb00748.x
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发表时间：
1994
期刊：
影响因子：
2
通讯作者：
Y. Lespinasse
中科院分区：
文献类型：
--
作者：
L. Bouvier;F. Fillon;Y. Lespinasse
在远系繁殖的物种如苹果中，单倍体植物在产生纯合材料的育种计划中可能特别有用。然而，必须有方法在单倍体植物中诱导染色体加倍。比较了秋水仙碱和安磺灵两种抗有丝分裂剂诱导苹果单倍体试管苗染色体加倍的效果。评价了三种秋水仙碱水平（0.025、0.25和1.25 mM）和三种安磺灵水平（5、15和30 μM）。三种技术也被使用和比较。测定存活率和染色体计数。观察到两种抗有丝分裂剂之间和三种技术之间的差异。本研究表明，安磺灵比秋水仙碱更适合于苹果单倍体试管苗的染色体加倍。
In an outbreeding species such as apple, haploid plants may be especially useful in breeding programmes for the production of homozygous material. However, methods must be available to induce chromosome doubling in the haploid plants. Two antimitotic agents, colchicine and oryzalin, were compared as regards their efficiency in inducing chromosome doubling of in vitro haploid apple shoots. Three colchicine levels (0.025, 0.25 and 1.25 mM) and three oryzalin levels (5, 15 and 30 μM) were evaluated. Three techniques were also used and compared. Survival rate and chromosome counts were determined. Differences were observed between the two antimitotic agents and between the three techniques. This study demonstrates that oryzalin could be a better choice than colchicine for chromosome doubling on haploid apple shoots in vitro.