LOX-1 mediates oxidized low-density lipoprotein-induced expression of matrix metalloproteinases in human coronary artery endothelial cells
LOX-1 mediates oxidized low-density lipoprotein-induced expression of matrix metalloproteinases in human coronary artery endothelial cells
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DOI：
10.1161/01.cir.0000047276.52039.fb
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发表时间：
2003-02-04
期刊：
影响因子：
37.8
通讯作者：
Mehta, JL
中科院分区：
文献类型：
--
作者：
Li, DY;Liu, L;Mehta, JL
氧化低密度脂蛋白（ox-LDL）在动脉粥样硬化区域的积聚可能增加斑块的不稳定性。氧化型低密度脂蛋白的积累和其凝集素样受体LOX-1的表达都已显示在动脉粥样硬化区域。本研究旨在检测LOX-1在人冠状动脉内皮细胞（HCAECs.Methods和Results-HCAECs）中对金属蛋白酶（MMP-1和MMP-3）的调节作用。Ox-LDL以浓度和时间依赖性方式增加MMP-1（胶原酶）和MMP-3（基质溶解素-1）的表达。Ox-LDL还增加胶原酶活性。氧化低密度脂蛋白没有显着影响金属蛋白酶的组织抑制剂的表达。天然LDL对MMPs的表达无影响。ox-LDL的作用是由其内皮受体LOX-1介导的，因为用LOX-1的阻断抗体（JTX 92，10 μ g/mL）预处理HCAEC可以阻止MMP对ox-LDL的反应（P
Background-Oxidized LDL (ox-LDL) accumulation in the atherosclerotic region may enhance plaque instability. Both accumulation of ox-LDL and expression of its lectin-like receptor, LOX-1, have been shown in atherosclerotic regions. This study was designed to examine the role of LOX-1 in the modulation of metalloprotemases (MMP-1 and MMP-3) in human coronary artery endothelial cells (HCAECs).Methods and Results-HCAECs were incubated with ox-LDL (10 to 80 mug/mL) for I to 24 hours. Ox-LDL increased the expression of MMP-1 (collagenase) and MMP-3 (stromelysin-1) in a concentration- and time-dependent manner. Ox-LDL also increased collagenase activity. Ox-LDL did not significantly affect the expression of tissue inhibitors of metalloproteinases. Native LDL had no effect on the expression of MMPs. The effects of ox-LDL were mediated by its endothelial receptor, LOX-1, because pretreatment of HCAECs with a blocking antibody to LOX-1 (JTX92, 10 mug/mL) prevented the expression of MMPs in response to ox-LDL (P