Structural mapping of protein interactions reveals differences in evolutionary pressures correlated to mRNA level and protein abundance
Structural mapping of protein interactions reveals differences in evolutionary pressures correlated to mRNA level and protein abundance
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DOI：
10.1016/j.str.2007.09.010
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发表时间：
2007-11-01
期刊：
影响因子：
5.7
通讯作者：
Kortemme, Tanja
中科院分区：
文献类型：
--
作者：
Eames, Matt;Kortemme, Tanja
酿酒酵母的全基因组研究得出结论，蛋白质进化速率的主要决定因素是表达水平：高表达的蛋白质通常进化得最慢。为了确定这种约束如何影响蛋白质相互作用的进化，我们直接测量蛋白质界面，表面和核心残基的结构映射域的相互作用，酵母基因组的进化速率。我们发现，mRNA水平和蛋白质丰度，虽然相关，报告的压力影响区域的蛋白质不同。与mRNA水平成比例的压力减缓了所有结构区域的进化速率，并减少了界面和其他表面之间速率差异的变异性。相比之下，一个域内的进化速率变化与蛋白质丰度的相关性要小得多。不同的压力可能主要与蛋白质生产的成本（mRNA水平）和功能（蛋白质丰度）利益相关。低mRNA水平蛋白质的界面可能具有更高的进化灵活性，并可能构成新功能的原材料。
Genome-wide studies in Saccharomyces cerevisiae concluded that the dominant determinant of protein evolutionary rates is expression level: highly expressed proteins generally evolve most slowly. To determine how this constraint affects the evolution of protein interactions, we directly measure evolutionary rates of protein interface, surface, and core residues by structurally mapping domain interactions to yeast genomes. We find that mRNA level and protein abundance, though correlated, report on pressures affecting regions of proteins differently. Pressures proportional to mRNA level slow evolutionary rates of all structural regions and reduce the variability in rate differences between interfaces and other surfaces. In contrast, the evolutionary rate variation within a domain is much less correlated to protein abundance. Distinct pressures may be associated primarily with the cost (mRNA level) and functional (protein abundance) benefit of protein production. Interfaces of proteins with low mRNA levels may have higher evolutionary flexibility and could constitute the raw material for new functions.