Long-term repetitive sodium lauryl sulfate-induced irritation of the skin:: an in vivo study
Long-term repetitive sodium lauryl sulfate-induced irritation of the skin:: an in vivo study
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DOI：
10.1111/j.0105-1873.2005.00703.x
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发表时间：
2005-11-01
影响因子：
5.5
通讯作者：
Maibach, HI
中科院分区：
文献类型：
--
作者：
Branco, N;Lee, I;Maibach, HI
皮肤可通过调节适应局部刺激物。这项研究的重点是长期暴露于刺激物，并试图证明住宿。十二烷基硫酸钠（SLS）在3个浓度（0.025%至0.075%）下诱导刺激性接触性皮炎。蒸馏水、丙酮和空室作为对照。将实验化合物施用于7名健康志愿者的前臂，持续24小时，然后在103天内用新室替换，持续6个非连续周。通过视觉评分（红斑和干燥）和生物工程参数（经表皮失水量（TEWL）、电容、比色法和激光多普勒血流仪（LDF））对可能的调节进行量化。在接触期间出现了显著的红斑、干燥、TEWL、肤色反射率和LDF值升高。在重复暴露后，红斑、TEWL、皮肤颜色反射和LDF的反应立即增强。然而，刺激性皮肤变化并未持续。暴露停止后刺激性参数恢复至基线。末次暴露后，无持续刺激或适应的证据。研究结果没有记录在这些测试条件下长期重复刺激暴露后的持续调节或适应性低敏。应该开发替代模型来证明或反驳调节假说。
Skin may adapt to topical irritants through accommodation. This study focuses on long-term exposure to irritants and attempts to demonstrate accommodation. Sodium lauryl sulfate (SLS) induced irritant contact dermatitis at 3 concentrations (0.025% to 0.075%). Distilled water, acetone and an empty chamber served as controls. Experimental compounds were applied to forearms of 7 healthy volunteers for 24 hr before replacing by a fresh chamber for 6 non-consecutive weeks over 103 days. Possible accommodation was quantified by visual scoring (erythema and dryness) and by bioengineering parameters: transepidermal water loss (TEWL), capacitance, chromametry and laser Doppler flowmetry (LDF). Significant erythema, dryness, elevated TEWL, skin colour reflectance and LDF values occurred during the exposure periods. Upon repeat exposure, an immediate and augmented response in erythema, TEWL, skin colour reflectance and LDF developed. However, irritant skin changes were not sustained. Irritation parameters return to baseline after cessation of exposure. There was no evidence of sustained irritation or accommodation after the last exposure. Study findings do not document sustained accommodation or adaptive hyposensitivity after long-term repetitive irritant exposure under these test conditions. Alternative models should be developed to prove or disprove the accommodation hypothesis.