Possible contribution of pannexin channel to ATP-induced currents in vitro in vasopressin neurons isolated from the rat supraoptic nucleus
Possible contribution of pannexin channel to ATP-induced currents in vitro in vasopressin neurons isolated from the rat supraoptic nucleus
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DOI：
10.1016/j.brainres.2011.04.017
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发表时间：
2011-06-07
期刊：
影响因子：
2.9
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Ohbuchi, Toyoaki;Yokoyama, Toru;Ueta, Yoichi
视上核（SON）的大细胞神经分泌细胞（MNCs）释放精氨酸抗利尿素（AVP）受这些神经元的电活动控制。ATP通过激活嘌呤能P2X和P2Y受体在SON MNCs的调控中起着至关重要的作用。最近关于P2X受体和pannexin通道之间相互作用的报道为中枢神经系统的生理学提供了新的见解；然而，AVP神经元中泛联蛋白通道的功能尚未被评估。在本研究中，我们研究了泛联蛋白通道在atp诱导的SON AVP神经元反应中的可能作用。我们使用全细胞膜片钳技术对表达avp增强绿色荧光蛋白转基因的大鼠SON MNCs进行分离。连接蛋白通道阻滞剂卡贝诺洛酮和甲氟喹呈浓度依赖性抑制atp诱导电流，而连接蛋白通道阻滞剂氟芬那酸和镧则没有作用。多细胞逆转录聚合酶链反应实验证实AVP神经元中存在pannexin-1 mRNA。排除了atp激活的瞬时受体电位和酸敏感离子通道的参与。这些结果表明，SON AVP神经元的泛联蛋白通道参与了神经元活动的调节机制。(C) 2011 Elsevier B.V.版权所有
Release of arginine vasopressin (AVP) from magnocellular neurosecretory cells (MNCs) of the supraoptic nucleus (SON) is controlled by the electrical activity of these neurons. ATP plays a crucial role in the regulation of SON MNCs by activating the purinergic P2X and P2Y receptors. Recent reports of interaction between P2X receptors and pannexin channels have provided new insights into the physiology of the central nervous system; however, the function of pannexin channels has not been assessed in AVP neurons. In the present study, we examined the possible contribution of the pannexin channel in ATP-induced responses in SON AVP neurons. We used the whole-cell patch-clamp technique in isolated rat SON MNCs that express an AVP-enhanced green fluorescent protein transgene. The ATP-induced current was inhibited in a concentration-dependent manner by pannexin channel blockers carbenoxolone and mefloquine, whereas the connexin channel blockers flufenamic acid and lanthanum had no effect. Multi-cell reverse transcriptase-polymerase chain reaction experiments confirmed the existence of pannexin-1 mRNA in AVP neurons. The involvement of the ATP-activated transient receptor potential vanilloid and acid-sensing ion channels was excluded. These results suggest that pannexin channels in SON AVP neurons are involved in the regulatory mechanisms of neuronal activity. (C) 2011 Elsevier B.V. All rights reserved.