Real-time quantitative PCR of telomerase mRNA is useful for the differentiation of benign and malignant pancreatic disorders
Real-time quantitative PCR of telomerase mRNA is useful for the differentiation of benign and malignant pancreatic disorders
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DOI：
10.1097/00006676-200105000-00001
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发表时间：
2001-05-01
期刊：
影响因子：
2.9
通讯作者：
Friess, H
中科院分区：
文献类型：
--
作者：
B端chler, P;Conejo-Garcia, JR;Friess, H
端粒酶活性的存在已被认为是恶性组织的特异性和敏感性标志物，据报道在胰腺癌中阳性率高达95%。本研究将29例胰腺癌组织与36例慢性胰腺炎组织和21例正常对照进行端粒酶活性分析，并利用实时定量PCR分析端粒酶mRNA表达，研究端粒酶是否能更好地区分胰腺良恶性疾病。在TRAP检测中，35%（10 / 29）的胰腺癌样本、3%（1 / 36）的慢性胰腺炎样本和正常胰腺组织样本中端粒酶存在活性，实时荧光定量PCR分析显示50%（10 / 20）的正常样本、86%（31 / 36）的慢性胰腺炎样本和90%（26 / 29）的胰腺癌样本中端粒酶mRNA存在表达。然而，定量表达数据显示，慢性胰腺炎样本相对高于正常水平的增加为5.5（范围，3.5-8.6），胰腺癌样本相对高于正常水平的增加为23.9（范围，18.6-30.7）（p < 0.01）。端粒酶活性及端粒酶mRNA正常以上倍数升高与性别、患者年龄、肿瘤分期、肿瘤分级均无相关性。这些数据表明，使用TRAP检测端粒酶活性在区分良性和恶性胰腺疾病方面存在局限性。而端粒酶mRNA的实时定量PCR分析能够对胰腺癌、正常胰腺和慢性胰腺炎进行高度敏感的检测和鉴别，可能成为一种新的可靠、简便、有效的癌症诊断工具。
The presence of telomerase activity has been proposed as a specific and sensitive marker for malignant tissue, and positivity rates of up to 95% have been reported in pancreatic cancer. In the present study telomerase activity analysis was reevaluated in 29 pancreatic cancer tissues compared with 36 chronic pancreatitis tissues and 21 normal controls, and a study was made of whether malignant and benign pancreatic disorders can be better differentiated using a novel technique-real-time quantitative PCR analysis-analyzing telomerase mRNA expression. Telomerase activity was present in 35% (10 of 29) of pancreatic cancer samples, 3% (one of 36) of chronic pancreatitis samples, and none of the normal pancreatic tissue samples in the TRAP assay, Real-time quantitative PCR analysis revealed the presence of telomerase mRNA expression in 50% (10 of 20) of normal, 86% (31 of 36) of chronic pancreatitis, and 90% (26 of 29) of pancreatic cancer samples. How ever, quantification of the expression data revealed that the relative increase above normal was 5.5 (range, 3.5-8.6) for chronic pancreatitis and 23.9 (range, 18.6-30.7) for pancreatic cancer samples (p < 0.01). NO relationship was found between telomerase activity and the fold increase of telomerase mRNA above normal and gender, patient age, tumor stage, or tumor grade. These data indicate that detection of telomerase activity using the TRAP assay has limitations in differentiating benign and malignant pancreatic disorders. However, telomerase mRNA analysis by real-time quantitative PCR analysis allows a highly sensitive detection and differentiation of pancreatic cancer from normal pancreas and chronic pancreatitis and thereby may serve as a new reliable, easy, and effective diagnostic tool for cancer diagnosis.