RADIAL AND HORIZONTAL DEPLOYMENT OF CLONALLY RELATED CELLS IN THE PRIMATE NEOCORTEX - RELATIONSHIP TO DISTINCT MITOTIC LINEAGES
RADIAL AND HORIZONTAL DEPLOYMENT OF CLONALLY RELATED CELLS IN THE PRIMATE NEOCORTEX - RELATIONSHIP TO DISTINCT MITOTIC LINEAGES
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DOI：
10.1016/0896-6273(95)90036-5
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发表时间：
1995-08-01
期刊：
影响因子：
16.2
通讯作者：
RAKIC, P
中科院分区：
文献类型：
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作者：
KORNACK, DR;RAKIC, P
为了分析恒河猴新皮层的细胞谱系，我们使用重组逆转录病毒标记脑室区（VZ）的单个祖细胞，然后用组织化学方法测定其后代在皮层神经发生期间和之后的分布。标记细胞在VZ中的分布模式提示不对称和对称分裂的祖细胞共存，表明有丝分裂后和有丝分裂后的后代在皮层神经发生过程中都有产生。在皮层中，观察到几种不同的克隆分布模式，并解释如下：径向排列的克隆表明，不对称分裂的祖细胞产生顺序的“兄弟姐妹”，这些“兄弟姐妹”沿着共同的径向路径串联迁移到皮层。相比之下，在单个层内水平方向的克隆表明，对称分裂产生多个横向移位的祖细胞，这些祖细胞反过来同时产生“表兄弟”细胞，它们一致地迁移到同一皮质层。因此，我们提出不同的有丝分裂谱系，共存于猴子的VZ，产生不同的径向或层流模式的克隆部署，预示着成年新皮层的细胞学组织。
To analyze cell lineage in the rhesus monkey neocortex, we used recombinant retroviruses to label individual progenitor cells in the ventricular zone (VZ), then determined histochemically the distribution of their progeny during and after the period of cortical neurogenesis. Distribution patterns of labeled cells in the VZ suggested the coexistence of asymmetrically and symmetrically dividing progenitor cells, indicating that both postmitotic and mitotic progeny are produced during cortical neurogenesis. In the cortex, several distinct patterns of clonal distribution were observed and interpreted as follows: clones aligned radially suggested that asymmetrically dividing progenitors generate sequential ''siblings'' that migrate, in tandem, along a common radial path to the cortex. In contrast, clones oriented horizontally within a single lamina suggested that symmetric divisions produce multiple, laterally displaced progenitors which, in turn, simultaneously generate ''cousin'' cells that migrate, in concert, to the same cortical layer. Thus, we propose that different mitotic lineages, which coexist in the monkey VZ, produce distinct radial or laminar patterns of clone deployment that foreshadow the cytological organization of the adult neocortex.