Self- and cross-incompatibility in Asparagus officinalis and Asparagus densiflorus cv. Sprengeri

Self- and cross-incompatibility in Asparagus officinalis and Asparagus densiflorus cv. Sprengeri
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Asparagus officinalis 和 Asparagus densiflorus cv. 的自交不亲和性和交叉不亲和性。

DOI:
10.1139/b96-196
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发表时间:
1996
期刊:
影响因子:
--
通讯作者:
E. Camadro
E. Camadro
中科院分区:
--
文献类型:
--
作者:
O. Marcellán;E. Camadro

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芦笋(Asparagus officinalis L.)(off),是雌雄异株和生殖分离的相关观赏,雌雄同株的芦笋densifiorus(Kunth)Asparagus cv. Sprengeri(spr).由于后者是一个潜在的有价值的种质资源,一项研究开始,以确定杂交障碍。用2个off品种的32个单株和3个spr品种的17个单株进行种内和种间杂交。将部分授粉雌蕊固定并通过荧光显微镜检查。在某些基因型组合中,检测到不亲和反应:(i)off × off:在柱头组织中,(ii)spr × spr:在柱头表面和花柱中,和(iii)spr × off:在柱头表面,柱头组织中,和花柱中。虽然在大多数基因型组合中花粉管到达胚珠,但种子仅在种内杂交中产生。结果表明,两种类型的内部障碍正在起作用:交叉不亲和的位置,
Garden asparagus, Asparagus officinalis L. (off), is dioecious and reproductively isolated from a related ornamental, monoecious Asparagus densifiorus (Kunth) Jessop cv. Sprengeri (spr). Since the latter is a potentially valuable source of germplasm, a study was initiated to identify hybridization barriers. Intra- and inter-specific crosses were made using 32 plants of two commercial cultivars of off and 17 plants of three introductions of spr. Part of the pollinated pistils were fixed and examined via fluorescence microscopy. In some combinations of genotypes, incompatibility reactions were detected: (i) off × off: in the stigmatic tissue, (ii) spr × spr: on the stigmatic surface and in the style, and (iii) spr × off: on the stigmatic surface, in the stigmatic tissue, and in the style. Although pollen tubes reached the ovules in most combinations of genotypes, seeds were only produced in intraspecific crosses. It is concluded that two types of internal barriers are acting: cross-incompatibility at the po...