A leafless epiphytic orchid, Taeniophyllum glandulosum Blume (Orchidaceae), is specifically associated with the Ceratobasidiaceae family of basidiomycetous fungi
A leafless epiphytic orchid, Taeniophyllum glandulosum Blume (Orchidaceae), is specifically associated with the Ceratobasidiaceae family of basidiomycetous fungi
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DOI：
10.1007/s00572-019-00881-7
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发表时间：
2019-02
期刊：
影响因子：
3.9
通讯作者：
Kento Rammitsu;Takahiro Yagame;Yumi Yamashita;T. Yukawa;S. Isshiki;Y. Ogura‐Tsujita
中科院分区：
文献类型：
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作者：
Kento Rammitsu;Takahiro Yagame;Yumi Yamashita;T. Yukawa;S. Isshiki;Y. Ogura‐Tsujita
兰科无叶附生植物经历了一个形态变态，其中根具有含叶绿体的皮层细胞，并且是唯一的光合器官用于碳增益。所有的兰花种子萌发过程中完全依赖于菌根真菌的碳供应，这种菌根协会通常持续在成年植物。然而，我们的知识的菌根协会的无叶附生兰花仍然是有限的，和菌根协会的营养收购在这些兰花物种的贡献在很大程度上是未知的。本研究以68株成熟植株和17株幼苗为材料，对腺带褶兰（Taeniophyllum glandulosum）的菌根真菌进行了分子鉴定。共获得187个真菌内转录间隔区序列，其中99%被鉴定为Ceratobasidiaceae。基于97%的序列相似性，这些序列被分类为5个操作分类单位（OTU）。最常见的序列是OTU1，占所有Ceratobasidiaceae序列的91%，尽管检测到其他遗传学上不同的Ceratobasidiaceae真菌。这些结果表明T. glandulosumis与Ceratobasidiaceae的一个特定组特别相关。结果表明，在香樟幼苗中发现的菌根真菌均属于OTU 1型，在同一寄主树上的成株中也发现了OTU 1型。对13种寄主树种的菌根真菌进行了比较，并对T.在11个树种上，glandulosum与OTU 1的结合频率较高。总之，T.在兰花的整个生命周期中，这种特定的关联仍然存在，并在不同的宿主树种上发现。
Leafless epiphytes in the Orchidaceae undergo a morphological metamorphosis in which the root has chloroplast-containing cortical cells and is the sole photosynthetic organ for carbon gain. All orchids are entirely dependent on mycorrhizal fungi for their carbon supply during seed germination, and this mycorrhizal association generally persists in adult plants. However, our knowledge of the mycorrhizal association of leafless epiphytic orchids remains limited, and the contribution of the mycorrhizal association to nutrient acquisition in these orchid species is largely unknown. In this study, the mycorrhizal fungi of a leafless epiphytic orchid,Taeniophyllum glandulosum, were identified molecularly using 68 mature plants and 17 seedlings. In total, 187 fungal internal transcribed spacer sequences were obtained, of which 99% were identified as Ceratobasidiaceae. These sequences were classified into five operational taxonomic units (OTUs) based on 97% sequence similarity. The most frequent sequence was OTU1, which accounted for 91% of all Ceratobasidiaceae sequences, although other phylogenetically distinct Ceratobasidiaceae fungi were detected. These results show thatT. glandulosumis specifically associated with a particular group of Ceratobasidiaceae. All mycorrhizal fungi found inT. glandulosumseedlings belonged to OTU1, which was also found in adult plants on the same host tree. The mycorrhizal fungi from 13 host tree species were compared, andT. glandulosumwas preferentially associated with OTU1 on 11 tree species. In conclusion,T. glandulosumis specifically associated with Ceratobasidiaceae fungi and this specific association remains throughout the orchid life cycle and is found on divergent host tree species.