Multi- and transgenerational effects following early-life exposure of zebrafish to permethrin and coumarin 47: Impact on growth, fertility, behavior and lipid metabolism
Multi- and transgenerational effects following early-life exposure of zebrafish to permethrin and coumarin 47: Impact on growth, fertility, behavior and lipid metabolism
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DOI：
10.1016/j.ecoenv.2020.111348
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发表时间：
2020-12-01
影响因子：
6.8
通讯作者：
Keiter, Steffen H.
中科院分区：
文献类型：
--
作者：
Blanc, Melanie;Cormier, Bettie;Keiter, Steffen H.
环境压力引起的跨代效应对生态系统和人类健康构成威胁。然而，对化学品影响几代人生活结果的潜力的观察和了解仍然有限。本文研究了两种环境污染物香豆素47和氯菊酯对暴露斑马鱼及其子代的影响。香豆素47通常存在于个人护理产品和染料中，而氯菊酯被用作家庭和农业拟除虫菊酯杀虫剂/驱虫剂。斑马鱼（F0）在受精后28天之前的早期发育期间暴露于辐射，其后代（F1-F3）在未暴露的情况下繁殖。一方面，香豆素47诱导的效应表明没有多代毒性。另一方面，我们发现，斑马鱼幼虫的行为受到显着影响，暴露于氯菊酯在F1至F3代的一些差异取决于浓度。这表明神经或神经肌肉功能发生持续改变。此外，脂质组学分析表明，氯菊酯治疗与斑马鱼的溶血磷脂酰胆碱水平部分相关，这是神经发育的重要脂质。总的来说，这些结果强调了最广泛使用的拟除虫菊酯之一可以引发斑马鱼神经系统的长期，多代和可能的跨代变化。这些神经行为的变化反映了直接暴露于高浓度氯菊酯下观察到的效应，因此需要对效应遗传的机制进行更多的研究。
Transgenerational effects induced by environmental stressors are a threat to ecosystems and human health. However, there is still limited observation and understanding of the potential of chemicals to influence life outcomes over several generations. In the present study, we investigated the effects of two environmental contaminants, coumarin 47 and permethrin, on exposed zebrafish (FO) and their progeny (F1-F3). Coumarin 47 is commonly found in personal care products and dyes, whereas permethrin is used as a domestic and agricultural pyrethroid insecticide/insect repellent. Zebrafish (F0) were exposed during early development until 28 days post-fertilization and their progeny (F1-F3) were bred unexposed. On one hand, the effects induced by coumarin 47 suggest no multigenerational toxicity. On the other hand, we found that behavior of zebrafish larvae was significantly affected by exposure to permethrin in F1 to F3 generations with some differences depending on the concentration. This suggests persistent alteration of the neural or neuromuscular function. In addition, lipidomic analyses showed that permethrin treatment was partially correlated with lysophosphatidylcholine levels in zebrafish, an important lipid for neurodevelopment. Overall, these results stress out one of the most widely used pyrethroids can trigger long-term, multi- and possibly transgenerational changes in the nervous system of zebrafish. These neurobehavioral changes echo the effects observed under direct exposure to high concentrations of permethrin and therefore call for more research on mechanisms underlying effect inheritance.