4 °C preparation of ferrite nanoparticles having protein molecules immobilized on their surfaces
4 °C preparation of ferrite nanoparticles having protein molecules immobilized on their surfaces
复制标题
4℃制备表面固定有蛋白质分子的铁氧体纳米颗粒
DOI：
10.1063/1.1452207
复制
发表时间：
2002
影响因子：
3.2
通讯作者：
M. Abe
中科院分区：
文献类型：
--
作者：
K. Nishimura;M. Hasegawa;Y. Ogura;T. Nishi;K. Kataoka;H. Handa;M. Abe
在由水溶液合成颗粒的过程中，将蛋白酶或蛋白质胰蛋白酶固定在粒径为约8 nm的铁氧体（Fe 3 O 4-γ Fe 2 O3混合溶液）细颗粒的表面上。该过程在露天中在低至4 °C的温度和pH值为1.9的近中性条件下进行，这与大多数生物活性分子以及胰蛋白酶相容。 因此，该技术有利于制备具有固定在其表面上的生物分子的磁铁矿颗粒，其将用于利用磁分离技术的生物医学应用。
Trypsin, a proteolytic enzyme or a protein, was immobilized onto the surfaces of ferrite (a Fe3O4–γFe2O3 mixed solution) fine particles, ∼8 nm in size, during the process in which the particles were synthesized from an aqueous solution. The process was performed in the open air at a temperature as low as 4 °C and on near-neutral condition of pH⩽9, which is compatible with most of the bioactive molecules as well as trypsin. Therefore this technique is advantageous for preparing magnetite particles having biomolecules immobilized on their surfaces, which will be used for biomedical applications utilizing magnetic separation technique.