Neurotropic Astrovirus in Cattle with Nonsuppurative Encephalitis in Europe
Neurotropic Astrovirus in Cattle with Nonsuppurative Encephalitis in Europe
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DOI：
10.1128/jcm.01195-14
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发表时间：
2014-09-01
影响因子：
9.4
通讯作者：
Seuberlich, Torsten
中科院分区：
文献类型：
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作者：
Bouzalas, Ilias G.;Wuethrich, Daniel;Seuberlich, Torsten
脑炎是一种常见的诊断条件牛神经系统疾病。许多受影响的动物在大脑中呈现非化脓性炎症反应模式。虽然这种模式支持病毒病因学，但在大部分病例中致病病原体仍然未知。利用病毒宏基因组学，我们在患有非化脓性脑炎的牛的大脑中鉴定了一种星状病毒（牛星状病毒[BoAstV]-CH 13）。此外，BoAstV RNA检测与逆转录-PCR和原位杂交在约四分之一（5/22动物）的牛与不明病因的非化脓性脑炎。病毒RNA主要在神经元和病理部位发现。这些发现支持BoAstV感染是牛脑炎的常见原因的观点。在系统发生学上，BoAstV-CH 13与来自动物脑炎病例和人类患者的罕见星状病毒分离株密切相关。未来的研究需要针对这些嗜神经性星状病毒的致病机制，流行病学和潜在的跨物种传播。
Encephalitis is a frequently diagnosed condition in cattle with neurological diseases. Many affected animals present with a non-suppurative inflammatory reaction pattern in the brain. While this pattern supports a viral etiology, the causative pathogen remains unknown in a large proportion of cases. Using viral metagenomics, we identified an astrovirus (bovine astrovirus [BoAstV]-CH13) in the brain of a cow with nonsuppurative encephalitis. Additionally, BoAstV RNA was detected with reverse transcription-PCR and in situ hybridization in about one fourth (5/22 animals) of cattle with nonsuppurative encephalitis of unknown etiology. Viral RNA was found primarily in neurons and at the site of pathology. These findings support the notion that BoAstV infection is a common cause of encephalitis in cattle. Phylogenetically, BoAstV-CH13 was closely related to rare astrovirus isolates from encephalitis cases in animals and a human patient. Future research needs to be directed toward the pathogenic mechanisms, epidemiology, and potential cross-species transmission of these neurotropic astroviruses.