Autoradiographic demonstration of a zonal distribution of 3H-dopamine-derived radioactivity in the mouse adrenal medulla perfusion-fixed with glutaraldehyde.
Autoradiographic demonstration of a zonal distribution of 3H-dopamine-derived radioactivity in the mouse adrenal medulla perfusion-fixed with glutaraldehyde.
复制标题
放射自显影显示用戊二醛灌注固定的小鼠肾上腺髓质中 3H-多巴胺衍生放射性的区域分布。
DOI：
10.1679/aohc1950.40.107
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
R. Coupland
中科院分区：
文献类型：
--
作者：
T. Hirano;S. Kobayashi;C. Kent;R. Coupland
用光镜和电镜放射自显影技术研究了小鼠肾上腺髓质染色质细胞中3h -多巴胺衍生放射性的定位。7只小鼠腹腔注射20 μCi/gbw同位素3,4 -二羟基（环g- 3h）盐酸苯乙胺，注射后15min ~ 24hrs用2.5%戊二醛从左心室灌注固定。浸泡后，通过乙醇系列脱水和Epon包埋，处理肾上腺和肝脏切片，使用浸渍法进行放射自显影。肾上腺髓质的A细胞和N细胞在注射同位素30min后放射性均达到最大值。注射后15 ~ 30min， A细胞的放射性明显高于N细胞。然而，两种类型的染色质细胞在稍后的时间点含有相似的放射性。结果表明，在所有时间点，A细胞和N细胞的3h -多巴胺衍生的放射性在皮质-髓质交界处下方的区域最高，而肾上腺髓质的中心部分的放射性总是较低。重标记组与轻标记组的细胞超微结构无明显差异。肝小叶中3h -多巴胺衍生的放射性无区域差异。3h -亮氨酸（l -leucine- 4,5 - 3h）注射后的小鼠肾上腺髓质放射自显影未见放射性的地带分布。这些结果与以下观点一致：小鼠肾上腺皮质-髓质交界处下方区域的染色质细胞比肾上腺髓质中心区域的染色质细胞具有更大的多巴胺处理能力，并且这种现象是多巴胺的特征。
Localization of 3H-dopamine-derived radioactivity in the chromaffin cells of the mouse adrenal medulla was studied by means of light and electron microscopic autoradiography. Twenty μCi/gbw of the isotope 3, 4-dihydroxy (ring-G-3H) phenylethylamine hydrochloride was injected intraperitoneally in seven mice, which were then perfusionfixed from the left ventricle of the heart with 2.5% glutaraldehyde from 15min to 24hrs after injection. Following the post-osmication, dehydration through an ethanol series and Epon embedding, sections of pieces of the adrenal gland and liver were processed for autoradiography using the dipping method.Both A and N cells of the adrenal medulla showed maximum radioactivity at 30min after injection of the isotope. The radioactivity on the A cells was remarkably higher than that on the N cells at 15 to 30min after the injection. However, both types of chromaffin cells contained a similar amount of radioactivity at later time points.It was demonstrated that at all time points examined, both A and N cells were highest in the 3H-dopamine-derived radioactivity in the zone immediately beneath the cortico-medullary junction, whereas the radioactivity in the central portion of the adrenal medulla was always lower. No difference was demonstrated between the ultrastructure of the heavily labeled chromaffin cells and that of the lightly labeled cells.No regional difference in the 3H-dopamine-derived radioactivity was demonstrated in the liver lobules.No zonal distribution of radioactivity was demonstrated in autoradiograms of the mouse adrenal medulla prepared after the 3H-leucine (L-leucine-4, 5-3H) injection.These results are consistent with the idea that the chromaffin cells in the zone immediately beneath the cortico-medullary junction of the mouse adrenal gland have a greater dopamine-handling capacity than those in the central portion of the adrenal medulla and that this phenomenon is characteristic of dopamine.