RECEPTORS FOR INTERLEUKIN-1 (ALPHA AND BETA) IN MOUSE-BRAIN - MAPPING AND NEURONAL LOCALIZATION IN HIPPOCAMPUS
RECEPTORS FOR INTERLEUKIN-1 (ALPHA AND BETA) IN MOUSE-BRAIN - MAPPING AND NEURONAL LOCALIZATION IN HIPPOCAMPUS
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DOI：
10.1016/0306-4522(91)90412-h
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发表时间：
1991-01-01
期刊：
影响因子：
3.3
通讯作者：
HAOUR, F
中科院分区：
文献类型：
--
作者：
BAN, E;MILON, G;HAOUR, F
使用人重组[I-125]白细胞介素-1-α和[I-125]白细胞介素-1-β作为配体，通过定量放射自显影技术对小鼠脑中的白细胞介素-1受体进行定位和表征。 两种配体提供相同的受体图谱。 就特异性而言，白细胞介素 1-α 和白细胞介素 1-β 在与 [I-125]白细胞介素-1-α 或 [I-125]白细胞介素-1-β 的结合竞争测定中同样有效（EC50 11 pM）。这些受体被证明高度集中在齿状回、大脑不同级别的脉络丛、垂体和脑垂体中。 脑膜。 它们在皮层中的浓度也很低，但在其他大脑结构中检测不到。 在齿状回中，当在浸有核乳剂的载玻片上观察时，白细胞介素-1 受体位于颗粒层和分子层（颗粒细胞）上。 使用秋水仙碱的选择性损伤来评估白细胞介素-1 受体的细胞定位。 海马区神经元被秋水仙碱破坏后，[I-125]白细胞介素 1 结合完全丧失，这表明白细胞介素 1 受体位于颗粒细胞上。 损伤后，稀疏的下层细胞，具有神经胶质细胞形态，也显示出显着的标记。 总之，白细胞介素-1受体位于小鼠齿状回的颗粒细胞上。 因此，这些神经元可能是白细胞介素 1 神经调节的目标，并且它们可能在白细胞介素 1 的中枢作用以及大脑免疫反应的控制中发挥关键作用。
Interleukin-1 receptors were mapped and characterized in mouse brain by quantitative autoradiography using human recombinant [I-125]interleukin-1-alpha and [I-125]interleukin-1-beta as ligands. Both ligands provide identical receptor mapping. In terms of specificity, interleukin-1-alpha and interleukin-1-beta were equally potent in binding competitions assays with either [I-125]interleukin-1-alpha or [I-125]interleukin-1-beta (EC50 11 pM).These receptors were shown to be highly concentrated in the dentate gyrus, in the choroid plexus at various levels of the brain, in the pituitary and in the meninges. They were also present at low concentrations in the cortex but undetectable in other brain structures. In the dentate gyrus, interleukin-1 receptors were localized on the granular and molecular layers (granule cells) when visualized on slides dipped in nuclear emulsion. Cellular localization of interleukin-1 receptors was assessed using selective lesion by colchicine. The complete loss of [I-125]interleukin-1 binding in hippocampal areas where neurons were destroyed by colchicine demonstrates that interleukin-1 receptors are located on granule cells. Following lesion, sparse understroyed cells, with glial cell morphology, also showed significant labelling.In conclusion, interleukin-1 receptors are located on the granule cells in the mouse dentate gyrus. These neurons may therefore be targets for neuromodulation by interleukin-1 and they may play a key role in the central effect of interleukin-1 as well as in the control of the immune response by the brain.