MOUSE T-CELL ANTIGEN RECEPTOR - STRUCTURE AND ORGANIZATION OF CONSTANT AND JOINING GENE SEGMENTS ENCODING THE BETA-POLYPEPTIDE
MOUSE T-CELL ANTIGEN RECEPTOR - STRUCTURE AND ORGANIZATION OF CONSTANT AND JOINING GENE SEGMENTS ENCODING THE BETA-POLYPEPTIDE
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DOI：
10.1016/0092-8674(84)90444-6
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
HOOD, L
中科院分区：
文献类型：
--
作者：
MALISSEN, M;MINARD, K;HOOD, L
胚系连接(J)基因片段和编码β基因的恒定(C)基因。在一个粘粒克隆上分离了小鼠T细胞抗原受体的链。有两个恒定的基因，C.beta1和C.beta2，每个都与一簇J.beta相关。基因片段。测定了C.beta2基因和J.beta2簇基因片段的核苷酸序列。C.beta2基因的编码序列与C.beta.1基因编码的c[互补]DNA克隆的序列非常相似。该基因有4个外显子，外显子-内含子结构与蛋白质的功能结构域没有明显的对应关系。J-beta2基因片段簇包含6个功能J基因片段。分离了C.beta.1、C.beta2、J.beta1和J.beta2区域的特异性探针，以检测T淋巴细胞的DNA重排。DNA重排可在两种J.beta中发生。基因片段簇，以及两个C.beta。基因似乎是有功能的。
The germ-line joining (J) gene segments and constant (C) genes encoding the .beta. chain of the mouse T cell antigen receptor were isolated on a single cosmid clone. There are 2 constant genes, C.beta.1 and C.beta.2, each associated with a cluster of J.beta. gene segments. The nucleotide sequences of the C.beta.2 gene and of the J.beta.2 cluster gene segments were determined. The coding sequence of the C.beta.2 gene is very similar to the sequence of a c[complementary]DNA clone encoded by the C.beta.1 gene. The C.beta.2 gene has 4 exons; exon-intron structure does not obviously correspond to the functional domains of the protein. The J.beta.2 gene segment cluster contains 6 functional J gene segments. Specific probes were isolated for the C.beta.1, C.beta.2, J.beta.1 and J.beta.2 regions to examine DNA rearrangements in T lymphocytes. DNA rearrangements can occur in both J.beta. gene segment clusters, and both C.beta. genes appear functional.