LARGE-SCALE PREPARATION OF BOVINE RENAL GLOMERULAR BASEMENT-MEMBRANE IN PRESENCE OF PROTEASE INHIBITORS
LARGE-SCALE PREPARATION OF BOVINE RENAL GLOMERULAR BASEMENT-MEMBRANE IN PRESENCE OF PROTEASE INHIBITORS
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DOI：
10.1080/00327487808069061
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发表时间：
1978-01-01
期刊：
影响因子：
--
通讯作者：
HUDSON, BG
中科院分区：
文献类型：
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作者：
FREYTAG, JW;OHNO, M;HUDSON, BG
描述了一种从牛肾小球中分离大量基底膜的方法，该方法在最大限度地减少或防止组织相关蛋白酶降解的条件下进行。该方法包括使用中-高浓度的酶抑制剂：EDTA、β-氨基己酸、N-乙基马来酰亚胺和DFP，并保持0-4℃的温度。C在整个过程中。肾小球制剂通过改进的筛分技术进行分离，通常纯度大于97%。在最佳条件下，在安全、快速的条件下，1wk即可分离出3~4种基底膜。
A method is described for the isolation of large quantities of basement membrane from bovine renal glomeruli under conditions which minimize or prevent degradation by tissue-associated proteases. The method incorporates the use of moderately-high concentrations of the protease inhibitors: EDTA, .epsilon.-amino caproic acid, N-ethylmaleimide and DFP and the maintenance of a temperature of 0-4.degree. C throughout the procedure. Glomeruli preparations are isolated by a modified sieving technique and are routinely of purity greater than 97%. Under optimum conditions, 3-4 of basement membrane can be isolated under safe and rapid conditions in 1 wk.