Possibility of drug-distribution measurement in the hair of drowned bodies: evaluation of drug stability in water-soaked hair using micro-segmental analysis
Possibility of drug-distribution measurement in the hair of drowned bodies: evaluation of drug stability in water-soaked hair using micro-segmental analysis
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DOI：
10.1007/s00414-022-02900-3
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发表时间：
2022-10-15
影响因子：
2.1
通讯作者：
Iwata, Yuko T.
中科院分区：
文献类型：
--
作者：
Kuwayama, Kenji;Miyaguchi, Hajime;Iwata, Yuko T.
在尸检中，对头发进行药物分析是揭示吸毒史的有效方法。此外，以前还开发了一种使用毛发估计死亡日期的方法，该方法是通过分析以0.4毫米为间隔分割的单根毛发(微段毛发分析)。然而，对于溺水的身体，头发中的药物可能会因为长时间浸泡在水中而被冲走。为了评估药物在溺水身体头发中分布的可能性，研究了药物在不同水溶液中浸泡的头发样品中的药物稳定性。首先，制备沿发干方向一致的含有特定药物的吸毒者的参考发丝。参与者在采集头发前每天服用4种花粉热药物(非索非那定、依匹斯汀、西替利嗪和氯雷他定)，持续约4个月。每个参照链被分成区域，每个区域被浸泡在含有不同溶质的不同溶液中，延长时间长达约2个月。在不含二价离子(Ca~(2+)和Mg~(2+))的溶液中，随着盐浓度的增加和浸泡时间的延长，头发中的药物含量下降了约5%。然而，在含有二价离子的溶液中，药物含量的下降可以忽略不计，这意味着二价离子阻止了头发中含有的药物被洗掉。由于天然河流和海水中含有二价离子，即使浸泡头发2个月，头发中的药物也很难被洗掉。因此，基于微段分析的药物分布测量方法同样适用于溺水身体的毛发检测。
In postmortem examinations, the drug analysis of hair is effective for revealing drug-use history. Additionally, a method to estimate the day of death using hair was previously developed by analyzing a single hair strand segmented at 0.4-mm intervals (micro-segmental hair analysis). However, for drowned bodies, drugs in the hair may be washed out due to soaking in water for extended periods. To evaluate the possibility of measuring drug distribution in the hair of drowned bodies, drug stability in hair samples soaked in various aqueous solutions was examined. First, reference hair strands of drug users containing specific drugs consistently along the hair shaft were prepared. The participants ingested 4 hay-fever medicines (fexofenadine, epinastine, cetirizine, and loratadine) every day for approximately 4 months before hair collection. Each reference strand was divided into regions, and each region was soaked in different solutions containing various solutes for extended periods up to approximately 2 months. In solutions without divalent ions (Ca2+ and Mg2+), the drug content in the hair decreased up to approximately 5 % with increasing salt concentration and soaking time. However, the decreased drug content was negligible in solutions containing divalent ions, implying that the divalent ions prevented drugs contained in hair from washing out. As natural river and sea waters contain divalent ions, the drugs in hair were hardly washed out even when the hair was soaked for 2 months. Thus, it was concluded that drug-distribution measurements using micro-segmental analysis can also be applied to the hairs of drowned bodies.