Isolation and characterization of a hormone-producing cell line from human small cell anaplastic carcinoma of the lung.
Isolation and characterization of a hormone-producing cell line from human small cell anaplastic carcinoma of the lung.
复制标题
从人小细胞肺癌中分离和鉴定产生激素的细胞系。
DOI：
--
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
E. Zimmerman
中科院分区：
文献类型：
--
作者：
O. Pettengill;C. Faulkner;D. Wurster‐Hill;L. Maurer;G. Sorenson;A. G. Robinson;E. Zimmerman
建立了肺小细胞间变性癌骨髓转移的连续细胞培养细胞系。通过光镜和电镜观察培养物的特征，在患者肿瘤的切片材料和细胞培养物中都观察到不寻常的同心细胞排列。这些细胞有两种特化的细胞连接，并含有神经内分泌细胞中描述的分泌样颗粒。乳酸脱氢酶同工酶模式与正常人血清相同，核型显示存在几个标记染色体。免疫过氧化物酶技术和放射免疫测定显示，抗利尿激素存在于细胞中，并在没有神经physin的情况下分泌到培养基中。细胞中也没有催产素。
A continuous cell culture line was established from a bone marrow metastasis of small cell anaplastic carcinoma of the lung. The cultures were characterized by light and electron microscopy, and an unusual concentric arrangement of cells was observed, both in sectioned material from the patient's tumor and from the cell cultures. The cells had two types of specialized cell junctions and contained secretory-like granules of the type described in neuroendocrine cells. Lactic dehydrogenase isozyme patterns were the same as those observed in normal human serum, and the karyotype revealed the presence of several marker chromosomes. Vasopressin was present in the cells and secreted into the culture medium in the absence of neurophysin, as shown by the immunoperoxidase technique and radioimmunoassay. Oxytocin was also absent from cells.