Fluorometric determination of glucose based on a redox reaction between glucose and aminopropyltriethoxysilane and in-situ formation of blue-green emitting silicon nanodots
Fluorometric determination of glucose based on a redox reaction between glucose and aminopropyltriethoxysilane and in-situ formation of blue-green emitting silicon nanodots
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DOI：
10.1007/s00604-018-3189-4
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发表时间：
2019-02-01
影响因子：
5.7
通讯作者：
Li, Zhaohui
中科院分区：
文献类型：
--
作者：
Cai, Qiyong;Meng, Hongmin;Li, Zhaohui
描述了一种用于荧光检测葡萄糖的方法。它是基于发现硅纳米点（SNDs）形成葡萄糖和氨丙基三乙氧基硅烷（APTES）在温和的实验条件下。由此形成的SND具有约2nm的平均直径，表现出良好的亲水性，蓝色荧光（在410/475 nm处具有激发/发射最大值），宽的pH耐受性，并且是光稳定的。该方法具有灵敏度高、特异性好、检测范围宽（10 M ~ 0.9 mM）等特点，可用于葡萄糖的定量分析。应用于加标血清中葡萄糖的测定，结果令人满意。
A method is described for fluorometric detection of glucose. It is based on the finding that silicon nanodots (SNDs) are formed from glucose and aminopropyltriethoxysilane (APTES) under mild experimental conditions. The SNDs thus formed have an average diameter of approximate to 2nm, exhibit good water dispersibility, blue fluorescence (with excitation/emission maxima at 410/475nm), broad pH tolerance, and are photostable. The assay was applied to the quantification of glucose with high sensitivity, good specificity, and over a wide detection range (from 10M to 0.9mM). It was applied to the determination of glucose in spiked serum samples and gave satisfactory results and recoveries.