IP3 receptor type 3 and PLCβ2 are co-expressed with taste receptors T1R and T2R in rat taste bud cells
IP3 receptor type 3 and PLCβ2 are co-expressed with taste receptors T1R and T2R in rat taste bud cells
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DOI：
10.1093/chemse/26.3.259
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发表时间：
2001-03-01
期刊：
影响因子：
3.5
通讯作者：
Emori, Y
中科院分区：
文献类型：
--
作者：
Asano-Miyoshi, M;Abe, K;Emori, Y
在脊椎动物的味觉系统中，许多促味剂都能激活Ca 2+信号级联。最近，我们已经表明，G（i2）和磷脂酶C β 2（PLC β 2）在味蕾细胞的一个子集中共表达，并可能参与大鼠味觉信号的Ca 2+触发。我们在这里报告，作为PLC β 2的下游成分，肌醇1，4，5-三磷酸（IP 3）受体（IP（3）R3）的3型同种型在大鼠味蕾中与PLC β 2相同的细胞中特异性表达。我们还发现，表达rT 2 R9（一种可能的放线菌酮受体）的细胞也包括在PLC β 2和IP（3）R3阳性细胞中，就像rT 1 R2（一种不同类型的味觉受体）一样。我们的研究结果表明，PLC β 2和IP（3）R3与G（i2）共同定位在味蕾细胞中，作为两种不同类型的味觉受体T1 R和T2 R的下游成分。
The Ca2+ signaling cascade has been reported to be activated by many tastants in vertebrate taste systems. Recently we have shown that G(i2) and phospholipase C beta2 (PLC beta2) are co-expressed in a subset of taste bud cells and are possibly involved in Ca2+ triggering of taste signaling in rats. We report here that, as a component downstream of PLC beta2, the type 3 isoform of the inositol 1,4,5-trisphosphate (IP3) receptor (IP(3)R3) is specifically expressed in the same cells as PLC beta2 in rat taste buds. We also show that cells expressing rT2R9, a probable cycloheximide receptor, are included among PLC beta2- and IP(3)R3-positive cells, as in the case of rT1R2, a different type of taste receptor. Our findings indicate that PLC beta2 and IP(3)R3 co-localize together with G(i2) as downstream components of two different types of taste receptors, T1R and T2R, in taste bud cells.