In vivo quantification of ethanol kinetics in rat brain
In vivo quantification of ethanol kinetics in rat brain
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DOI：
10.1038/sj.npp.1301023
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发表时间：
2006-12-01
影响因子：
7.6
通讯作者：
Pfefferbaum, Adolf
中科院分区：
文献类型：
--
作者：
Adalsteinsson, Elfar;Sullivan, Edith V.;Pfefferbaum, Adolf
在3 T下使用质子磁共振波谱测量大鼠腹腔内（i. p.）或胃内（i.g.）酒精注射，并估计急性酒精对脑代谢产物的影响。观察持续时间为1-1.5小时，酒精采样的时间分辨率范围从4s-4分钟。所观察到的时间过程酒精脑浓度遵循一致的模式，其特征在于快速吸收，中间分布，和较慢的清除率，接近线性衰减。在8只健康Wistar大鼠的样本中，线性清除率项的截距（外推至注射时间）与每单位瘦体重的给药剂量相关性良好。将基于光谱测量清除率的酒精浓度估计值与观察结束时血液样本的血液酒精水平进行比较，结果与给药剂量一致。连续质子光谱测量提供了一种有效的体内方法，用于真实的时间定量脑酒精摄取和消除动力学。
Proton magnetic resonance spectroscopy was used at 3T to measure the uptake and clearance of brain ethanol in rats after bolus intraperitoneal (i.p.) or intragastric (i.g.) alcohol injection, and to estimate the effects of acute alcohol on brain metabolites. The observation duration was 1-1.5 h with temporal resolution of alcohol sampling ranging from 4s-4 min. The observed time course of alcohol brain concentration followed a consistent pattern characterized by a rapid absorption, an intermediate distribution, and a slower clearance that approached a linear decay. In a sample of eight healthy Wistar rats, the intercept of the linear clearance term, extrapolated back to the time of injection, correlated well with the administered dose per unit of lean body mass. Alcohol concentration estimation based on spectroscopically measured clearance was compared with blood alcohol levels from blood samples at the end of observation, and were in good agreement with the administered dose. Serial proton spectroscopy measurements provide a valid in vivo method for quantifying brain alcohol uptake and elimination kinetics in real time.